Benchikha Naima, Messaoudi Mohammed, Larkem Imane, Ouakouak Hamza, Rebiai Abdelkrim, Boubekeur Siham, Ferhat Mohamed Amine, Benarfa Adel, Begaa Samir, Benmohamed Mokhtar, Almasri Diena M, Hareeri Rawan H, Youssef Fadia S
Laboratory of Applied Chemistry and Environment (LCAE), Chemistry Department, University of Hamma Lakhdar El-Oued, B.P.789, El-Oued 39000, Algeria.
Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera, Djelfa 17200, Algeria.
Life (Basel). 2022 Oct 11;12(10):1579. doi: 10.3390/life12101579.
L. is commonly used in folk medicine to treat hypertension and diabetes and to heal wounds. The present work aimed to evaluate the different biological activities of hydroalcoholic extract, its total phenol and flavonoid content, and its mineral elements. Results showed that extract showed significant antioxidant potential in 2-diphenyl-1-picrylhydrazyl (DPPH) assay with IC equal to 8.68 μg/mL but with moderate activity in galvinoxyl assay with IC of 21.82 μg/mL and mild activity in the -carotene assay. It also showed a pronounced anti-hyperglycemic activity using α-amylase inhibitory assay (IC = 111.68 µg/mL) and exceeds that of acarbose. showed excellent activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with IC values of 28.69 and 4.93 μg/mL, respectively, postulating its promising anti-Alzheimer potential. The plant extract exhibited a strong anti-inflammatory effect with Bovine Serum Albumin (BSA) denaturation inhibitory potential estimated by 97.53% at 2 mg/mL, which was further confirmed by the in vivo carrageen-induced edema model. The extract revealed its richness in flavonoids and phenols, evidenced by its polyphenols content (36.35 ± 0.294 μg GAE/mg) and flavonoids (24.30 ± 0.44 μg QE/mg). It is rich in minerals necessary for human health, such as calcium, potassium, iron, sodium, magnesium, manganese and zinc. Molecular docking performed for previously identified compounds on human -amylase, 5-lipoxygenase (5-LOX) and acetylcholine esterase confirmed the results. Thus, it can be concluded that can be a good candidate for alleviating many health-debilitating problems and can be highly beneficial in the pharmaceutical industry and medical research.
L. 在民间医学中常用于治疗高血压、糖尿病和伤口愈合。本研究旨在评估水醇提取物的不同生物活性、其总酚和黄酮含量以及其矿物质元素。结果表明，提取物在2-二苯基-1-苦基肼（DPPH）测定中显示出显著的抗氧化潜力，IC值等于8.68μg/mL，但在加尔文氧基测定中活性中等，IC值为21.82μg/mL，在β-胡萝卜素测定中活性较弱。它在α-淀粉酶抑制试验（IC = 111.68μg/mL）中还显示出明显的抗高血糖活性，超过了阿卡波糖。显示出对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）具有优异的活性，IC值分别为28.69和4.93μg/mL，推测其具有潜在的抗阿尔茨海默病潜力。植物提取物表现出强烈的抗炎作用，在2mg/mL时通过牛血清白蛋白（BSA）变性抑制潜力估计为97.53%，这在体内角叉菜胶诱导的水肿模型中得到了进一步证实。提取物显示出其富含黄酮类化合物和酚类，其多酚含量（36.35±0.294μg GAE/mg）和黄酮类化合物（24.30±0.44μg QE/mg）证明了这一点。它富含人体健康所需的矿物质，如钙、钾、铁、钠、镁、锰和锌。对先前鉴定的化合物在人α-淀粉酶、5-脂氧合酶（5-LOX）和乙酰胆碱酯酶上进行的分子对接证实了结果。因此，可以得出结论，L. 可以成为缓解许多健康衰弱问题的良好候选物，并且在制药行业和医学研究中可能非常有益。
