Bharathi Devaraj, Thiruvengadam Nandagopal Jaya Ganesh, Lee Jintae, Ranjithkumar Rajamani
School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of Korea.
Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
Polymers (Basel). 2023 Jun 16;15(12):2700. doi: 10.3390/polym15122700.
In the treatment of bacterial contamination, the problem of multi-drug resistance is becoming an increasingly pressing concern. Nanotechnology advancements enable the preparation of metal nanoparticles that can be assembled into complex systems to control bacterial and tumor cell growth. The current work investigates the green production of chitosan functionalized silver nanoparticles (CS/Ag NPs) using and their inhibition efficacy against bacterial pathogens and lung cancer cells (A549). Initially, a brown color formation confirmed the synthesis, and the chemical nature of the synthesized NPs were examined by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). FTIR demonstrated the occurrence of CS and functional groups in the synthesized CS/Ag NPs. The electron microscopy study exhibited CS/Ag NPs with a spherical morphology and size ranges of 6-45 nm, while XRD analysis demonstrated the crystallinity of Ag NPs. Further, the bacterial inhibition property of CS/Ag NPs was examined against and , which showed clear inhibition zones at different concentrations. In addition, the antibacterial properties were further confirmed by a fluorescent AO/EtBr staining technique. Furthermore, prepared CS/Ag NPs exhibited a potential anti-cancer character against a human lung cancer cell line (A549). In conclusion, our findings revealed that the produced CS/Ag NPs could be used as an excellent inhibitory material in industrial and clinical sectors.
在细菌污染的治疗中，多重耐药性问题正变得越来越紧迫。纳米技术的进步使得制备金属纳米颗粒成为可能，这些纳米颗粒可以组装成复杂系统来控制细菌和肿瘤细胞的生长。当前的工作研究了使用[具体物质未给出]绿色生产壳聚糖功能化银纳米颗粒（CS/Ag NPs）及其对细菌病原体和肺癌细胞（A549）的抑制效果。最初，棕色的形成证实了合成，通过紫外可见光谱、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）结合能量色散光谱（EDS）以及透射电子显微镜（TEM）对合成的纳米颗粒的化学性质进行了研究。FTIR表明在合成的CS/Ag NPs中存在壳聚糖和[具体物质未给出]的官能团。电子显微镜研究显示CS/Ag NPs具有球形形态，尺寸范围为6 - 45纳米，而X射线衍射分析证明了银纳米颗粒的结晶性。此外，研究了CS/Ag NPs对[具体细菌未给出]和[具体细菌未给出]的细菌抑制特性，在不同浓度下显示出清晰的抑菌圈。另外，通过荧光AO/EtBr染色技术进一步证实了抗菌性能。此外，制备的CS/Ag NPs对人肺癌细胞系（A549）表现出潜在的抗癌特性。总之，我们的研究结果表明，所制备的CS/Ag NPs可作为工业和临床领域优秀的抑制材料。
