Catanese B, Lagana A, Marino A, Picollo R, Rotatori M
Pharmacol Res Commun. 1986 Apr;18(4):385-403. doi: 10.1016/0031-6989(86)90091-3.
A method for the extraction and quantification of benzydamine and its metabolite N-oxide by liquid chromatography with fluorescence detection in plasma samples is described. This method has adequate sensitivity, specificity and is reproducible. The use of the extraction column allowed a recovery of both benzydamine and its metabolite of over 97% to be obtained. The plasma levels of benzydamine and its metabolite N-oxide were studied after oral administration as sugar-coated tablets or topical application to the vaginal mucosa as a cream to 6 healthy volunteers. After topical application, the plasma concentrations of the unchanged drug and its metabolite are lower than those obtained following oral administration. These data further stress the concept that, whenever possible, topical use should be considered the treatment of choice since, along with a more selective therapy, the incidence of systemic side effects can be considerably reduced.
描述了一种采用液相色谱-荧光检测法从血浆样本中提取并定量测定苄达明及其代谢产物N-氧化物的方法。该方法具有足够的灵敏度、特异性且可重现。使用萃取柱可使苄达明及其代谢产物的回收率均超过97%。对6名健康志愿者口服糖衣片或阴道黏膜局部涂抹乳膏形式的苄达明后，研究了其血浆水平及代谢产物N-氧化物的情况。局部涂抹后，原形药物及其代谢产物的血浆浓度低于口服给药后的浓度。这些数据进一步强调了这样一个概念，即只要有可能，应考虑将局部用药作为首选治疗方法，因为这样除了能实现更具选择性的治疗外，还可大幅降低全身副作用的发生率。
