Adedokun Folake Latifat, Ajayi Abraham, Essiet Utibeima Udo, Oduyebo Oyin, Adeleye Adeyemi Isaac, Smith Stella Ifeanyi
Department of Microbiology, University of Lagos, Akoka, Nigeria.
Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.
Microbiol Insights. 2023 Jun 23;16:11786361231181909. doi: 10.1177/11786361231181909. eCollection 2023.
Multidrug resistance and invasiveness of non-typhoidal (NTS) serovars have in recent times brought to the fore the public health risk associated with salmonellosis. This study was aimed at profiling NTS serovars isolated from food animals and humans for their susceptibility to antibiotics and plasmid replicon types. Forty seven NTS serovars were profiled for their susceptibility to antibiotics using the disk diffusion method. Polymerase chain reaction based replicon typing assay was used for profiling plasmid replicon types detected in isolates. High rate of resistance were found for amoxicillin/clavulanic acid (40/47; 85.1%), cefuroxime (38/47; 80.9%) and ceftazidime (30/47; 63.8%). Thirty one (65.9%) and 33 (70.2%) showed intermediate resistance to ofloxacin and ciprofloxacin respectively. Plasmids of sizes ranging from 14.3 to 16.7 kb were detected in 24 (51.1%) of isolates with some serovars harbouring multiple plasmids. FIA, FIB, Frep and W plasmid replicon types were detected in 11, 4, 2 and 1 of the isolates respectively. Three of the isolates harboured both FIA and FIB replicon types. The high rate of resistance to β-lactams observed in serovars harbouring different plasmid replicon types in this study highlight potential public health threat and the need for prudent use of antibiotics in human and veterinary medicine.
非伤寒（NTS）血清型的多重耐药性和侵袭性最近凸显了与沙门氏菌病相关的公共卫生风险。本研究旨在分析从食用动物和人类中分离出的NTS血清型对抗生素的敏感性以及质粒复制子类型。使用纸片扩散法分析了47种NTS血清型对抗生素的敏感性。基于聚合酶链反应的复制子分型测定用于分析分离株中检测到的质粒复制子类型。发现阿莫西林/克拉维酸（40/47；85.1%）、头孢呋辛（38/47；80.9%）和头孢他啶（30/47；63.8%）的耐药率较高。31株（65.9%）和33株（70.2%）分别对氧氟沙星和环丙沙星表现出中度耐药。在24株（51.1%）分离株中检测到大小在14.3至16.7 kb之间的质粒，一些血清型携带多个质粒。分别在11株、4株、2株和1株分离株中检测到FIA、FIB、Frep和W质粒复制子类型。其中3株分离株同时携带FIA和FIB复制子类型。本研究中观察到携带不同质粒复制子类型的血清型对β-内酰胺类抗生素的高耐药率凸显了潜在的公共卫生威胁以及在人类和兽医学中谨慎使用抗生素的必要性。
