Russo Ilaria, Bencardino Daniela, Napoleoni Maira, Andreoni Francesca, Schiavano Giuditta Fiorella, Baldelli Giulia, Brandi Giorgio, Amagliani Giulia
Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, Italy.
Istituto Zooprofilattico Sperimentale dell'Umbria e Delle Marche "Togo Rosati", 06126 Perugia, Italy.
Antibiotics (Basel). 2022 May 28;11(6):725. doi: 10.3390/antibiotics11060725.
Nontyphoidal salmonellosis (NTS) is the second most commonly reported gastrointestinal infection in humans and an important cause of food-borne outbreaks in Europe. The use of antimicrobial agents for animals, plants, and food production contributes to the development of antibiotic-resistant strains that are transmissible to humans through food. The aim of this study was to investigate the presence and the potential dissemination of multidrug-resistant (MDR) strains isolated in the Marche Region (Central Italy) via the food chain. Strains were isolated from different sources: food, human, food animal/livestock, and the food-processing environment. Among them, we selected MDR strains to perform their further characterization in terms of resistance to tetracycline agent, carriage of genes, and plasmid profiles. Tetracycline resistance genes were detected by PCR and plasmid replicons by PCR-based replicon typing (PBRT). A total of 102 MDR strains were selected among the most prevalent serovars: . Infantis (n = 36/102), . Derby (n = 20/102), . Typhimurium (n = 18/102), and a monophasic variant of . Typhimurium (MVST, n = 28/102). Resistance to sulfisoxazole (86%) and tetracycline (81%) were the most common, followed by ampicillin (76%). FIIS was the most predominant replicon (17%), followed by FII (11%) and FIB (11%) belonging to the IncF incompatibility group. Concerning the characterization of genes, B was the most frequently detected (27/89), followed by A (10/89), G (5/89), and M (1/89). This study showed the potential risk associated with the MDR strains circulating along the food chain. Hence, epidemiological surveillance supported by molecular typing could be a very useful tool to prevent transmission of resistant from food to humans, in line with the One Health approach.
非伤寒沙门氏菌病（NTS）是人类报告的第二常见胃肠道感染，也是欧洲食源性疾病暴发的重要原因。在动物、植物和食品生产中使用抗菌剂会导致产生对抗生素耐药的菌株，这些菌株可通过食物传播给人类。本研究的目的是调查意大利中部马尔凯地区分离出的多重耐药（MDR）菌株通过食物链的存在情况和潜在传播情况。菌株从不同来源分离：食品、人类、食用动物/牲畜以及食品加工环境。其中，我们选择MDR菌株，以便在对四环素类药物的耐药性、基因携带情况和质粒图谱方面进行进一步表征。通过PCR检测四环素耐药基因，通过基于PCR的复制子分型（PBRT）检测质粒复制子。在最常见的血清型中总共选择了102株MDR菌株：婴儿沙门氏菌（n = 36/102）、德比沙门氏菌（n = 20/102）、鼠伤寒沙门氏菌（n = 18/102）以及鼠伤寒沙门氏菌的单相变体（MVST，n = 28/102）。对磺胺异恶唑（86%）和四环素（81%）的耐药最为常见，其次是氨苄青霉素（76%）。FIIS是最主要的复制子（17%），其次是属于IncF不相容群的FII（11%）和FIB（11%）。关于基因的表征，B是最常检测到的（27/89），其次是A（10/89）、G（5/89）和M（1/89）。本研究表明了与食物链中传播的MDR菌株相关的潜在风险。因此，按照“同一健康”方法，由分子分型支持的流行病学监测可能是预防耐药菌从食物传播给人类的非常有用的工具。
