Zhao Gang, Xie Lu, Wu Yan, Wang Bing, Teng Weilin, Sun Zhou, Kao Qingjun, Liu Wei, Pi Xionge, Ma Haiyan
Department of Infectious Disease Control and Prevention, Hangzhou Center for Disease Control and Prevention, Hangzhou, China.
School of Public Health, Hangzhou Normal University, Hangzhou, China.
Front Microbiol. 2023 Jun 12;14:989303. doi: 10.3389/fmicb.2023.989303. eCollection 2023.
Owing to urbanization, living habits have changed widely, leading to alterations in the intestinal microbiota of urban residents. However, there are few studies on the characteristics of intestinal microbiota of adolescents living in different urbanized areas in China.
A total of 302 fecal samples collected from adolescent students in eastern China were examined. 16S rRNA high-throughput sequencing was used to identify the fecal microbiota. These data were combined with questionnaire survey results to investigate the effect of urbanization on the intestinal microbiota of adolescents in eastern China. Moreover, the role of lifestyle habits in this relationship was also evaluated.
The results revealed significant differences in the structure of the intestinal microbiota among adolescents living in regions with different levels of urbanization. Adolescents living in urban regions had a significantly higher proportion of ( < 0.001, FDR = 0.004), whereas those living in towns and rural regions had higher proportions of ( < 0.001, FDR < 0.001) and ( < 0.05, FDR = 0.019). The diversity of the intestinal microbiota was higher in urban residents than in adolescents living in towns and rural regions ( < 0.05). In addition, the differences in intestinal microbiota across individuals living in cities, towns, and rural regions were related to dietary preferences, flavor preferences, and sleep and exercise durations. Adolescents who ate more meat had more (LDA = 3.622, = 0.04), while the abundance of - is higher among adolescents who ate more condiments (LDA = 4.285, = 0.02). The abundance of was significantly increased in adolescents with longer sleep durations (LDA = 4.066, 0.03). Adolescents who exercised for a long duration had more than those who exercised for a shorter duration (LDA = 4.303, = 0.04).
Our research has preliminarily demonstrated that there were differences in the composition of Gut microbiome in stool samples of adolescents living in different urbanized areas, and provide a scientific basis for the maintenance of a healthy intentional microbota in adolescences.
由于城市化进程，生活习惯发生了广泛变化，导致城市居民肠道微生物群发生改变。然而，关于中国不同城市化地区青少年肠道微生物群特征的研究较少。
对从中国东部青少年学生中收集的302份粪便样本进行检测。采用16S rRNA高通量测序来鉴定粪便微生物群。将这些数据与问卷调查结果相结合，以研究城市化对中国东部青少年肠道微生物群的影响。此外，还评估了生活方式习惯在这种关系中的作用。
结果显示，生活在不同城市化水平地区的青少年肠道微生物群结构存在显著差异。生活在城市地区的青少年 比例显著更高（ < 0.001，FDR = 0.004），而生活在城镇和农村地区的青少年 （ < 0.001，FDR < 0.001）和 （ < 0.05，FDR = 0.019）比例更高。城市居民肠道微生物群的多样性高于生活在城镇和农村地区的青少年（ < 0.05）。此外，城市、城镇和农村地区个体之间肠道微生物群的差异与饮食偏好、口味偏好以及睡眠和运动时长有关。吃肉较多的青少年 更多（LDA = 3.622， = 0.04），而吃调味品较多的青少年中 - 的丰度更高（LDA = 4.285， = 0.02）。睡眠时长较长的青少年中 的丰度显著增加（LDA = 4.066， 0.03）。运动时间长的青少年比运动时间短的青少年 更多（LDA = 4.303， = 0.04）。
我们的研究初步证明，生活在不同城市化地区的青少年粪便样本中肠道微生物组的组成存在差异，并为青少年维持健康的肠道微生物群提供了科学依据。
