Pi Xionge, Du Zhi, Teng Weilin, Fu Hao, Hu Lidan, Li Jiabin, Ding Jieying, Yang Xiaoxia, Zhang Yinjun
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Nutr. 2024 Aug 7;11:1411374. doi: 10.3389/fnut.2024.1411374. eCollection 2024.
Childhood obesity presents a serious health concern associated with gut microbiota alterations. Dietary interventions targeting the gut microbiota have emerged as promising strategies for managing obesity in children. This study aimed to elucidate the impact of stachyose (STS) supplementation on the gut microbiota composition and metabolic processes in obese children. Fecal samples were collected from 40 obese children (20 boys and 20 girls) aged between 6 and 15 and fermentation was conducted with or without the addition of STS, respectively, followed by 16S rRNA amplicon sequencing and analysis of short-chain fatty acids (SCFAs) and gases. Notably, our results revealed that STS supplementation led to significant alterations in gut microbiota composition, including an increase in the abundance of beneficial bacteria such as and , and a decrease in harmful bacteria including , , , and . Moreover, STS supplementation resulted in changes in SCFAs production, with significant increases in acetate levels and reductions in propionate and propionate, while simultaneously reducing the generation of gases such as HS, H, and NH. The Area Under the Curve (AUC)-Random Forest algorithm and PICRUSt 2 were employed to identify valuable biomarkers and predict associations between the gut microbiota, metabolites, and metabolic pathways. The results not only contribute to the elucidation of STS's modulatory effects on gut microbiota but also underscore its potential in shaping metabolic activities within the gastrointestinal environment. Furthermore, our study underscores the significance of personalized nutrition interventions, particularly utilizing STS supplementation, in the management of childhood obesity through targeted modulation of gut microbial ecology and metabolic function.
儿童肥胖是一个严重的健康问题，与肠道微生物群的改变有关。针对肠道微生物群的饮食干预已成为管理儿童肥胖的有前景的策略。本研究旨在阐明补充水苏糖（STS）对肥胖儿童肠道微生物群组成和代谢过程的影响。从40名6至15岁的肥胖儿童（20名男孩和20名女孩）中采集粪便样本，分别在添加或不添加STS的情况下进行发酵，然后进行16S rRNA扩增子测序，并分析短链脂肪酸（SCFA）和气体。值得注意的是，我们的结果显示，补充STS导致肠道微生物群组成发生显著变化，包括有益细菌如[具体细菌名称1]和[具体细菌名称2]的丰度增加，以及有害细菌如[具体细菌名称3]、[具体细菌名称4]、[具体细菌名称5]和[具体细菌名称6]的减少。此外，补充STS导致SCFA产生发生变化，乙酸盐水平显著增加，丙酸盐和丁酸盐减少，同时减少了如硫化氢、氢气和氨气等气体的产生。采用曲线下面积（AUC）-随机森林算法和PICRUSt 2来识别有价值的生物标志物，并预测肠道微生物群、代谢物和代谢途径之间的关联。这些结果不仅有助于阐明STS对肠道微生物群的调节作用，还强调了其在塑造胃肠道环境中代谢活动方面的潜力。此外，我们的研究强调了个性化营养干预的重要性，特别是利用补充STS，通过有针对性地调节肠道微生物生态和代谢功能来管理儿童肥胖。
