Department of Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Asian Pac J Cancer Prev. 2023 Jun 1;24(6):2089-2097. doi: 10.31557/APJCP.2023.24.6.2089.
Prostate cancer is one of the most widespread cancers in the world. Early diagnosis is the most important factor in treatment efficiency. Furthermore, new methods for early diagnosis and treatment play an important role. In this study, we designed targeted conjugation of antibodies with iron nanoparticles and evaluated the binding properties of antibodies to prostate cancers and benign tissues. This method in addition to having a lower cost has high sensitivity and specificity.
Anti- PSCA antibodies were purified and conjugated to super magnetic oxide nanoparticles (SPION). Then, iron staining on prostate adenocarcinoma tissues was performed. At the same time, immunohistochemically staining was performed on similar tissues to compare the results. In addition, benign prostatic hyperplasia (BPH) samples were used as a control sample.
In adenocarcinoma tissues with iron staining, many blue spots are seen compared to benign tissues, and the number of these spots increases with increasing tumor grade.
These findings indicate the characteristic of iron staining as a conjugate antibody to iron can be an appropriate approach to specific staining of tumor markers in cancer tissues and can be used to diagnose prostate cancer due to its safety, low cost, sensitivity, and specificity.
前列腺癌是世界上最常见的癌症之一。早期诊断是治疗效果最重要的因素。此外，新的早期诊断和治疗方法起着重要作用。在本研究中，我们设计了抗体与铁纳米粒子的靶向结合，并评估了抗体与前列腺癌和良性组织的结合特性。这种方法除了成本更低外，还具有更高的灵敏度和特异性。
纯化抗 PSCA 抗体并将其与超顺磁氧化铁纳米粒子（SPION）结合。然后对前列腺腺癌组织进行铁染色。同时，对类似组织进行免疫组织化学染色，以比较结果。此外，还使用良性前列腺增生（BPH）样本作为对照样本。
与良性组织相比，铁染色的腺癌组织中可见许多蓝色斑点，并且随着肿瘤分级的增加，这些斑点的数量增加。
这些发现表明，作为铁结合物的铁染色抗体具有特征性，可以作为肿瘤标志物在癌症组织中的特异性染色的适当方法，并且由于其安全性、低成本、灵敏度和特异性，可用于诊断前列腺癌。
