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生成用于 3D 体外建模的血管化人心肌类器官。

Generation of vascularized human cardiac organoids for 3D in vitro modeling.

机构信息

QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia.

QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, QLD 4000, Australia.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102371. doi: 10.1016/j.xpro.2023.102371. Epub 2023 Jun 28.

DOI:10.1016/j.xpro.2023.102371
PMID:37384522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10511847/
Abstract

Here, we provide a protocol for next-generation human cardiac organoid modeling containing markers of vascularized tissues. We describe steps for cardiac differentiation, harvesting cardiac cells, and generating vascularized human cardiac organoids. We then detail downstream analysis of functional parameters and fluorescence labeling of human cardiac organoids. This protocol is useful for high throughput disease modeling, drug discovery, and providing mechanistic insight into cell-cell and cell-matrix interactions. For complete details on the use and execution of this protocol, please refer to Voges et al. and Mills et al..

摘要

在这里，我们提供了一个包含血管组织标志物的下一代人类心脏类器官建模方案。我们描述了心脏分化、收获心脏细胞和生成血管化人类心脏类器官的步骤。然后，我们详细介绍了人类心脏类器官的功能参数和荧光标记的下游分析。该方案可用于高通量疾病建模、药物发现，并为细胞-细胞和细胞-基质相互作用提供机制见解。有关此方案使用和执行的完整详细信息，请参阅 Voges 等人和 Mills 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/1d9784f7c08a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/c763a8fa7840/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/739e1da8f489/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/ac03af870fad/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/37a3f0d8a040/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/1d9784f7c08a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/c763a8fa7840/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/739e1da8f489/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/ac03af870fad/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/37a3f0d8a040/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b91/10511847/1d9784f7c08a/gr4.jpg

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