Dinsmore C E, Maren T H
Teratology. 1986 Feb;33(1):85-91. doi: 10.1002/tera.1420330111.
Acetazolamide, a potent and highly specific inhibitor of carbonic anhydrase, is teratogenic in mammalian embryos and when administered during early limb development causes unique limb defects in a time- and dose-dependent manner. The regenerating urodele limb is often considered to be a good experimental analog of limb development and, if it employs the same mechanisms of tissue interactions during pattern formation, should be susceptible to teratogens which selectively disrupt developmental limb patterning. This study demonstrates that while carbonic anhydrase inhibition is toxic to the red-backed salamander, Plethodon cinereus, it does not have the same teratogenic effect on limb regeneration as seen in mammalian limb development. Several points are considered as to why the regenerating limb, at least in this salamander species, may not be suitable for studying this class of teratogen.
乙酰唑胺是一种强效且高度特异性的碳酸酐酶抑制剂，对哺乳动物胚胎具有致畸性，在肢体发育早期给药时，会以时间和剂量依赖的方式导致独特的肢体缺陷。再生的有尾两栖类肢体常被认为是肢体发育的良好实验模型，如果它在模式形成过程中采用相同的组织相互作用机制，应该会对选择性破坏肢体发育模式的致畸剂敏感。本研究表明，虽然碳酸酐酶抑制对红背蝾螈（Plethodon cinereus）有毒性，但对肢体再生并没有像在哺乳动物肢体发育中看到的那样具有相同的致畸作用。关于为什么再生肢体，至少在这种蝾螈物种中，可能不适合研究这类致畸剂，我们考虑了几个要点。
