Huang Ying, Luo Peng, Jiang Fu-Hui, Gao Hui-Ze, Cui Li-Fan, Zhao Zhe
Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China.
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510301, China.
Fish Shellfish Immunol Rep. 2023 Jun 21;5:100103. doi: 10.1016/j.fsirep.2023.100103. eCollection 2023 Dec 15.
Interleukins (ILs) are a subgroup of secreted cytokines, which are molecules involved in the intercellular regulation of the immune system. In this study, 12 IL homologs were cloned and functionally identified from obscure puffer , and they were termed as , and . Multiple alignment results showed that except for ToIL-24 and ToIL-27, other deduced ToIL proteins shared typical characteristics and structure with other known fish ILs. Phylogenetic analysis revealed that 12 ToILs were evolutionarily closely related to their counterparts in other selected vertebrates. Tissue distribution assay demonstrated that the mRNA transcripts of most genes were constitutively expressed in all tissues examined, with relatively high expression in immune tissues. Following and infection, the expression levels of 12 in the spleen and liver were significantly upregulated, and their response over time varied. Taken together, these data were discussed accordingly with the expression and the immune response under the different situations tested. The results suggest that the 12 genes are involved in the antibacterial immune response in .
白细胞介素（ILs）是分泌型细胞因子的一个亚群，这些细胞因子是参与免疫系统细胞间调节的分子。在本研究中，从暗纹东方鲀中克隆并功能鉴定了12个IL同源物，它们被命名为ToIL-1、ToIL-2、ToIL-3、ToIL-4、ToIL-5、ToIL-6、ToIL-7、ToIL-8、ToIL-9、ToIL-10、ToIL-24和ToIL-27。多重比对结果表明，除ToIL-24和ToIL-27外，其他推导的ToIL蛋白与其他已知鱼类ILs具有典型的特征和结构。系统发育分析表明，12个ToILs在进化上与其在其他选定脊椎动物中的对应物密切相关。组织分布分析表明，大多数ToIL基因的mRNA转录本在所有检测组织中组成性表达，在免疫组织中表达相对较高。在鳗弧菌和迟缓爱德华氏菌感染后，脾脏和肝脏中12个ToILs的表达水平显著上调，且它们随时间的反应有所不同。综上所述，根据不同测试情况下的ToIL表达和免疫反应对这些数据进行了相应讨论。结果表明，这12个ToIL基因参与了暗纹东方鲀的抗菌免疫反应。
