Department of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
Medical School of Chinese PLA, Beijing 100853, China.
World J Gastroenterol. 2023 Jun 14;29(22):3482-3496. doi: 10.3748/wjg.v29.i22.3482.
Due to the poor prognosis of gastric cancer (GC), early detection methods are urgently needed. Plasma exosomal circular RNAs (circRNAs) have been suggested as novel biomarkers for GC.
To identify a novel biomarker for early detection of GC.
Healthy donors (HDs) and GC patients diagnosed by pathology were recruited. Nine GC patients and three HDs were selected for exosomal whole-transcriptome RNA sequencing. The expression profiles of circRNAs were analyzed by bioinformatics methods and validated by droplet digital polymerase chain reaction. The expression levels and area under receiver operating characteristic curve values of plasma exosomal circRNAs and standard serum biomarkers were used to compare their diagnostic efficiency.
There were 303 participants, including 240 GC patients and 63 HDs, involved in the study. The expression levels of exosomal hsa_circ_0079439 were significantly higher in GC patients than in HDs ( < 0.0001). However, the levels of standard serum biomarkers were similar between the two groups. The area under the curve value of exosomal hsa_circ_0079439 was higher than those of standard biomarkers, including carcinoembryonic antigen, carbohydrate antigen (CA)19-9, CA72-4, alpha-fetoprotein, and CA125 (0.8595 0.5862, 0.5660, 0.5360, 0.5082, and 0.5018, respectively). The expression levels of exosomal hsa_circ_0079439 were significantly decreased after treatment ( < 0.05). Moreover, the expression levels of exosomal hsa_circ_0079439 were obviously higher in early GC (EGC) patients than in HDs ( < 0.0001).
Our results suggest that plasma exosomal hsa_circ_0079439 is upregulated in GC patients. Moreover, the levels of exosomal hsa_circ_0079439 could distinguish EGC and advanced GC patients from HDs. Therefore, plasma exosomal hsa_circ_0079439 might be a potential biomarker for the diagnosis of GC during both the early and late stages.
由于胃癌（GC）的预后较差，因此迫切需要早期检测方法。血浆外泌体环状 RNA（circRNA）已被提议作为 GC 的新型生物标志物。
寻找用于 GC 早期检测的新型生物标志物。
招募了健康供体（HD）和经病理诊断为 GC 的患者。对 9 名 GC 患者和 3 名 HD 进行了外泌体全转录组 RNA 测序。通过生物信息学方法分析 circRNA 的表达谱，并通过液滴数字聚合酶链反应进行验证。比较了血浆外泌体 circRNA 和标准血清生物标志物的表达水平和受试者工作特征曲线下面积值，以比较其诊断效率。
本研究共纳入 303 名参与者，包括 240 名 GC 患者和 63 名 HD。GC 患者的血浆外泌体 hsa_circ_0079439 的表达水平明显高于 HD（<0.0001）。然而，两组之间的标准血清生物标志物水平相似。外泌体 hsa_circ_0079439 的曲线下面积值高于包括癌胚抗原、糖链抗原（CA）19-9、CA72-4、甲胎蛋白和 CA125 在内的标准生物标志物（0.8595 vs 0.5862、0.5660、0.5360、0.5082 和 0.5018）。治疗后外泌体 hsa_circ_0079439 的表达水平明显降低（<0.05）。此外，早期 GC（EGC）患者的血浆外泌体 hsa_circ_0079439 表达水平明显高于 HD（<0.0001）。
我们的结果表明，GC 患者的血浆外泌体 hsa_circ_0079439 呈上调表达。此外，外泌体 hsa_circ_0079439 的水平可将 EGC 和晚期 GC 患者与 HD 区分开来。因此，血浆外泌体 hsa_circ_0079439 可能是 GC 早期和晚期诊断的潜在生物标志物。
