Wang Yuxuan, Xiong Jiali, Ouyang Kun, Ling Mingwang, Luo Junyi, Sun Jiajie, Xi Qianyun, Chen Ting, Zhang Yongliang
College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, China.
College of Medicine, Jiaxing University, Jiaxing, Zhejiang, China.
J Tissue Eng. 2025 Apr 30;16:20417314251319474. doi: 10.1177/20417314251319474. eCollection 2025 Jan-Dec.
Extracellular vesicles (EVs) have emerged as a promising strategy for treating a wide spectrum of pathologies, as they can deliver their cargo to recipient cells and regulate the signaling pathway of these cells to modulate their fate. Despite the great potential of EVs in clinical applications, their low yield and the challenges of cargo loading remain significant obstacles, hindering their transition from experimental research to clinical practice. Therefore, promoting EV release and enhancing EV cargo-loading are promising fields with substantial research potential and broad application prospects. In this review, we summarize the clinical applications of EVs, the methods and technologies for their large-scale production, engineering, and modification, as well as the challenges that must be addressed during their development. We also discuss the future perspectives of this exciting field of research to facilitate its transformation from bench to clinical reality.
细胞外囊泡（EVs）已成为一种有前景的治疗多种病症的策略，因为它们可以将其内容物递送至受体细胞，并调节这些细胞的信号通路以调控其命运。尽管EVs在临床应用中具有巨大潜力，但其低产量以及货物装载的挑战仍然是重大障碍，阻碍了它们从实验研究向临床实践的转化。因此，促进EVs释放和增强EVs货物装载是具有巨大研究潜力和广阔应用前景的有前景的领域。在本综述中，我们总结了EVs的临床应用、其大规模生产、工程化和修饰的方法及技术，以及其开发过程中必须解决的挑战。我们还讨论了这一令人兴奋的研究领域的未来前景，以促进其从实验室到临床实际的转化。
