Sun Ning, Chen Yong, Zhang Jiaxun, Cao Jin, Huang Hongjuan, Wang Jie, Guo Wentao, Li Xiaojun
Department of Clinical Laboratory Science, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Clinical Medicine Research Center, The Affiliated Suqian First People's Hospital of Nanjing Medical University, Suqian, China.
Front Microbiol. 2023 Jun 14;14:1185216. doi: 10.3389/fmicb.2023.1185216. eCollection 2023.
This study aimed to identify the bacterial composition in the pancreatic fluid of severe and critical acute pancreatitis (SAP and CAP) patients.
A total of 78 pancreatic fluid samples were collected from 56 SAP and CAP patients and analyze using aerobic culture and gene next-generation sequencing. The clinical data of the patients were obtained from the electronic medical records.
Among the total 78 samples, gene NGS identified a total of 660 bacterial taxa, belonging to 216 species in 123 genera. The dominant aerobic bacteria included , , and , while the dominant anaerobic bacteria included , , and . As compared to aerobic culturing, 95.96% (95/99) of the aerobic cultured bacteria were detected using the gene NGS.
The pancreatic infections in SAP and CAP patients might originate not only from the gut but also from the oral cavity and airways as well as related environments. Dynamic analysis of bacterial profile and abundance showed that some bacteria with low abundance might become the main pathogenic bacteria. There were no significant differences in the bacterial diversity between SAP and CAP.
本研究旨在确定重症和危重症急性胰腺炎（SAP和CAP）患者胰液中的细菌组成。
从56例SAP和CAP患者中收集了78份胰液样本，采用需氧培养和基因二代测序进行分析。患者的临床资料来自电子病历。
在总共78个样本中，基因二代测序共鉴定出660个细菌分类群，属于123个属的216个物种。主要的需氧菌包括 、 和 ，而主要的厌氧菌包括 、 和 。与需氧培养相比，基因二代测序检测到了95.96%（95/99）的需氧培养细菌。
SAP和CAP患者的胰腺感染可能不仅源于肠道，还源于口腔、气道以及相关环境。细菌谱和丰度的动态分析表明，一些低丰度细菌可能成为主要病原菌。SAP和CAP之间的细菌多样性没有显著差异。
