Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, D-60120 Heidelberg, Germany.
Sci Adv. 2023 Jun 30;9(26):eadh0721. doi: 10.1126/sciadv.adh0721.
RNA polymerase II initiates transcription either randomly or in bursts. We examined the light-dependent transcriptional activator White Collar Complex (WCC) of to characterize the transcriptional dynamics of the strong () promoter and the weaker () promoter. We show that WCC is not only an activator but also represses transcription by recruiting histone deacetylase 3 (HDA3). Our data suggest that bursts of transcription are governed by a long-lived refractory state established and maintained by WCC and HDA3 at the core promoter, whereas transcription of is determined by WCC binding dynamics at an upstream activating sequence. Thus, in addition to stochastic binding of transcription factors, transcription factor-mediated repression may also influence transcriptional bursting.
RNA 聚合酶 II 可以随机或爆发式地启动转录。我们研究了 的光依赖性转录激活因子 White Collar Complex（WCC），以表征强启动子 () 和较弱启动子 () 的转录动力学。我们表明，WCC 不仅是一种激活剂，还通过招募组蛋白去乙酰化酶 3（HDA3）来抑制转录。我们的数据表明，转录的爆发式活动受 WCC 和 HDA3 在核心启动子处建立和维持的长时程无反应状态控制，而转录则由 WCC 在启动子上游激活序列处的结合动力学决定。因此，除了转录因子的随机结合外，转录因子介导的抑制也可能影响转录爆发。
