Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16709-14. doi: 10.1073/pnas.1009474107. Epub 2010 Aug 31.
The photoreceptor and PAS/LOV protein VIVID (VVD) modulates blue-light signaling and influences light and temperature responses of the circadian clock in Neurospora crassa. One of the main actions of VVD on the circadian clock is to influence circadian clock phase by regulating levels of the transcripts encoded by the central clock gene frequency (frq). How this regulation is achieved is unknown. Here we show that VVD interacts with complexes central for circadian clock and blue-light signaling, namely the WHITE-COLLAR complex (WCC) and FREQUENCY-interacting RNA helicase (FRH), a component that complexes with FRQ to mediate negative feedback control in Neurospora. VVD interacts with FRH in the absence of WCC and FRQ but does not seem to control the exosome-mediated negative feedback loop. Instead, VVD acts to modulate the transcriptional activity of the WCC.
光感受器和 PAS/LOV 蛋白 VIVID（VVD）调节蓝光信号，并影响 Neurospora crassa 生物钟的光和温度反应。VVD 对生物钟的主要作用之一是通过调节中央时钟基因频率（frq）编码的转录本水平来影响生物钟相位。这种调节是如何实现的尚不清楚。在这里，我们表明 VVD 与生物钟和蓝光信号的中央复合物相互作用，即 WHITE-COLLAR 复合物（WCC）和 FREQUENCY-interacting RNA 解旋酶（FRH），后者是与 FRQ 复合物以介导 Neurospora 中负反馈控制的组件。VVD 在没有 WCC 和 FRQ 的情况下与 FRH 相互作用，但似乎并不控制外切体介导的负反馈环。相反，VVD 作用是调节 WCC 的转录活性。
