Gao Yao, Fan Xue, Zhang Xuechen, Guan Qinglin, Xing Yongheng, Song Wenbo
College of Chemistry, Jilin University, Changchun, 130012, PR China.
College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, 116029, PR China.
Biosens Bioelectron. 2023 Oct 1;237:115483. doi: 10.1016/j.bios.2023.115483. Epub 2023 Jun 24.
For high-performance dual-photoelectrode assay, developing a pair of photoactive materials with well-matched band structure and the design of a powerful sensing strategy are highly desirable. Herein, the Zn-TBAPy pyrene-based MOF and BiVO/TiC Schottky junction were employed as photocathode and photoanode to form an efficient dual-photoelectrode system. The integration of the cascaded hybridization chain reaction (HCR)/DNAzyme-assisted feedback amplification with DNA walker-mediated cycle amplification strategy realizes femtomolar HPV16 dual-photoelectrode bioassay. Through the activation of the HCR cascaded with the DNAzyme system in the presence of HPV16, plentiful HPV16 analogs are generated that leads to exponential positive feedback signal amplification. Meanwhile on the Zn-TBAPy photocathode, the NDNA hybridizes with the bipedal DNA walker followed by circular cleavage by Nb.BbvCI NEase, producing a dramatically enhanced PEC readout. The achieved ultralow detection limit of 0.57 fM and a wide linear range of 10 nM-10 nM showcase the excellent performance of the developed dual-photoelectrode system.
对于高性能双光电极检测，开发一对具有良好匹配能带结构的光活性材料以及设计一种强大的传感策略是非常必要的。在此，基于芘的Zn-TBAPy金属有机框架（MOF）和BiVO/TiC肖特基结被用作光阴极和光阳极，以形成一个高效的双光电极系统。级联杂交链式反应（HCR）/DNA酶辅助反馈放大与DNA步行者介导的循环放大策略相结合，实现了飞摩尔级的HPV16双光电极生物检测。在HPV16存在的情况下，通过激活与DNA酶系统级联的HCR，产生了大量的HPV16类似物，从而导致指数级正反馈信号放大。同时，在Zn-TBAPy光阴极上，NDNA与双足DNA步行者杂交，随后被Nb.BbvCI核酸内切酶进行环状切割，产生显著增强的光电化学（PEC）读数。所实现的0.57飞摩尔的超低检测限和10纳摩尔至10纳摩尔的宽线性范围展示了所开发的双光电极系统的优异性能。
