Fenwick D C, Blackshaw J K, Green D J
Vet Res Commun. 1986 Jul;10(4):309-15. doi: 10.1007/BF02213994.
Twenty three non pregnant, non lactating aged Merino and Border Leicester ewes were subject to four jugular venipunctures one hour apart (times 0, 1, 2 and 3). Four different delay intervals of 12.5, 64.0, 128.0 and 252.9 seconds between catching and sampling (delays 1, 2, 3 and 4) were used on each sheep in a random manner. Estimates were made of packed cell volumes (PCVs), plasma and erythrocyte sodium and potassium concentrations. The mean PCV at time 0 of 35.4 per cent was significantly higher than the levels of 33.1, 31.8 and 32.2 per cent at times 1, 2 and 3 respectively. There was no significant difference between the means at times 1, 2 and 3. The mean plasma potassium concentrations at time 0 of 4.37 mmol/l was significantly higher than the levels of 4.16 and 4.14 mmol/l at times 1 and 3 respectively. Delays had no significant effects on the means of any of the parameters studied. The findings strongly suggest that sheep become excited by initial handling but not by subsequent venipunctures and that a delay between catching and sampling of between 12.5 and 252.9 second has no effect on the blood constituents studied.
23只未怀孕、未哺乳的成年美利奴羊和边境莱斯特母羊，每隔一小时进行四次颈静脉穿刺（时间点0、1、2和3）。每只羊随机采用捕捉与采样之间12.5、64.0、128.0和252.9秒这四种不同的延迟间隔（延迟1、2、3和4）。对血细胞压积（PCV）、血浆以及红细胞钠和钾浓度进行了测定。时间点0时的平均血细胞压积为35.4%，显著高于时间点1、2和3时分别为33.1%、31.8%和32.2%的水平。时间点1、2和3时的平均值之间无显著差异。时间点0时的平均血浆钾浓度为4.37 mmol/l，显著高于时间点1和3时分别为4.16 mmol/l和4.14 mmol/l的水平。延迟对所研究的任何参数的平均值均无显著影响。这些发现有力地表明，羊在最初处理时会变得兴奋，但后续的静脉穿刺不会使其兴奋，并且捕捉与采样之间12.5至252.9秒的延迟对所研究的血液成分没有影响。
