Heard D J, Huft V J
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610-0126, USA.
J Zoo Wildl Med. 1998 Mar;29(1):14-7.
The effects of short-term physical restraint and isoflurane anesthesia on hematologic and serum biochemistry parameters were evaluated in 12 island flying foxes (Pteropus hypomelanus). Physical restraint was associated with significantly decreased calcium, cholesterol, globulin, albumin, hemoglobin concentrations, red blood cell count, and hematocrit and increased glucose, phosphorus, and potassium concentrations. Isoflurane restraint was associated with significantly decreased calcium, cholesterol, albumin, globulin, and glucose concentrations, aspartate transaminase and alkaline phosphatase activities, white blood cell count, red blood cell count, hemoglobin, hematocrit, and total neutrophil and lymphocyte counts and significantly increased phosphorus and chloride concentrations. For those variables where both restraint forms produced an alteration (red blood cell count, hemoglobin, hematocrit, calcium, phosphorus, glucose), the magnitude of change was always significantly greater for the physically restrained animals. This study indicates that in adult flying foxes isoflurane anesthesia is preferable to physical restraint for collection of blood samples for hematologic and plasma biochemical analysis. Additionally, blood samples should be collected as soon as possible after capture, regardless of restraint technique.
在12只海岛狐蝠（黑首狐蝠）身上评估了短期身体约束和异氟烷麻醉对血液学及血清生化参数的影响。身体约束与钙、胆固醇、球蛋白、白蛋白、血红蛋白浓度、红细胞计数和血细胞比容显著降低以及葡萄糖、磷和钾浓度升高有关。异氟烷约束与钙、胆固醇、白蛋白、球蛋白和葡萄糖浓度、天冬氨酸转氨酶和碱性磷酸酶活性、白细胞计数、红细胞计数、血红蛋白、血细胞比容以及总中性粒细胞和淋巴细胞计数显著降低以及磷和氯浓度显著升高有关。对于两种约束方式均产生改变的变量（红细胞计数、血红蛋白、血细胞比容、钙、磷、葡萄糖），身体约束的动物其变化幅度总是显著更大。本研究表明，对于采集血液样本进行血液学和血浆生化分析而言，在成年狐蝠中，异氟烷麻醉比身体约束更可取。此外，无论采用何种约束技术，捕获后应尽快采集血样。
