Nooranidoost Mohammad, Cogan N G, Stoodley Paul, Gloag Erin S, Hussaini M Yousuff
Department of Mathematics, Florida State University, Tallahassee, FL, USA.
Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
Biofilm. 2023 Jun 3;5:100133. doi: 10.1016/j.bioflm.2023.100133. eCollection 2023 Dec.
biofilms are relevant for a variety of disease settings, including pulmonary infections in people with cystic fibrosis. Biofilms are initiated by individual bacteria that undergo a phenotypic switch and produce an extracellular polymeric slime (EPS). However, the viscoelastic characteristics of biofilms at different stages of formation and the contributions of different EPS constituents have not been fully explored. For this purpose, we develop and parameterize a mathematical model to study the rheological behavior of three biofilms - wild type PAO1, isogenic rugose small colony variant (RSCV), and mucoid variant biofilms against a range of experimental data. Using Bayesian inference to estimate these viscoelastic properties, we quantify the rheological characteristics of the biofilm EPS. We employ a Monte Carlo Markov Chain algorithm to estimate these properties of variant biofilms in comparison to those of wild type. This information helps us understand the rheological behavior of biofilms at different stages of their development. The mechanical properties of wild type biofilms change significantly over time and are more sensitive to small changes in their composition than the other two mutants.
生物膜与多种疾病情况相关，包括囊性纤维化患者的肺部感染。生物膜由经历表型转换并产生细胞外聚合黏液（EPS）的单个细菌引发。然而，生物膜在不同形成阶段的粘弹性特征以及不同EPS成分的贡献尚未得到充分探索。为此，我们开发并参数化了一个数学模型，以研究三种生物膜——野生型PAO1、同基因皱缩小菌落变体（RSCV）和黏液变体生物膜——针对一系列实验数据的流变行为。使用贝叶斯推理来估计这些粘弹性特性，我们量化了生物膜EPS的流变特征。与野生型相比，我们采用蒙特卡洛马尔可夫链算法来估计变体生物膜的这些特性。这些信息有助于我们了解生物膜在其发育不同阶段的流变行为。野生型生物膜的力学性能随时间显著变化，并且比其他两个突变体对其组成的微小变化更敏感。
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