Alipour Nasim, Fallahnezhad Somaye, Bagheri Javad, Babaloo Hamideh, Tahmasebi Fatemeh, Sazegar Ghasem, Haghir Hossein
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Iran J Basic Med Sci. 2023;26(7):805-811. doi: 10.22038/IJBMS.2023.69668.15171.
Diabetes during gestation is one of the most common pregnancy complications and has adverse effects on offspring, including a negative impact on the offspring's central nervous system (CNS). Diabetes is a metabolic disease associated with visual impairment. Due to the importance of the lateral geniculate body (LGB) in the visual pathway, the present study examined the effect of maternal diabetes on the expression of gamma-aminobutyric acid (GABA and GABA) and metabotropic Glutamate (mGlu2) receptors in the LGB of male neonates of diabetic rats.
Diabetes was induced in female adult rats by a single intraperitoneal dose of streptozotocin (STZ) 65 (mg/kg). In the Insulin-treated diabetic rats, diabetes was controlled by subcutaneous NPH-insulin injection daily. After mating and delivery, male offspring were killed by carbon dioxide gas inhalation at P0, P7, and P14 (postnatal days 0, 7, and 14). The expression of GABA, GABA, and mGluR2 in the LGB of male neonates was determined using the immunohistochemistry (IHC) method.
The expression of GABA and GABA was significantly reduced, whereas the expression of mGluR2 was markedly increased in the diabetic group compared with the control and insulin-treated groups at P0, P7, and P14.
The results of the present study showed that induction of diabetes altered the expression of GABA, GABA, and mGluR2 in the LGB of male neonates born to diabetic rats at P0, P7, and P14. Moreover, insulin treatment could reverse these effects of diabetes.
妊娠期糖尿病是最常见的妊娠并发症之一，会对后代产生不利影响，包括对后代中枢神经系统（CNS）产生负面影响。糖尿病是一种与视力损害相关的代谢性疾病。由于外侧膝状体（LGB）在视觉通路中的重要性，本研究检测了母体糖尿病对糖尿病大鼠雄性新生子代LGB中γ-氨基丁酸（GABA）和代谢型谷氨酸受体（mGlu2）表达的影响。
通过腹腔注射单次剂量65mg/kg链脲佐菌素（STZ）诱导成年雌性大鼠患糖尿病。对于胰岛素治疗的糖尿病大鼠，通过每日皮下注射中性鱼精蛋白锌胰岛素来控制糖尿病。交配和分娩后，在出生后第0天（P0）、第7天（P7）和第14天（P14），通过吸入二氧化碳气体处死雄性子代。采用免疫组织化学（IHC）方法测定雄性新生子代LGB中GABA、GABA和mGluR2的表达。
与对照组和胰岛素治疗组相比，糖尿病组在P0、P7和P14时，GABA和GABA的表达显著降低，而mGluR2的表达明显增加。
本研究结果表明，糖尿病的诱导改变了糖尿病大鼠雄性新生子代在P0、P7和P14时LGB中GABA、GABA和mGluR2的表达。此外，胰岛素治疗可逆转糖尿病的这些影响。
