Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran; Department of Anatomical Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
J Chem Neuroanat. 2023 Oct;132:102326. doi: 10.1016/j.jchemneu.2023.102326. Epub 2023 Aug 22.
This study investigates the impact of maternal diabetes on the expression of α2-adrenergic and M2 muscarinic receptors in the primary visual cortex of male offspring born to diabetic rats.
In adult female rats, a single dose of intraperitoneal streptozotocin (STZ) was used to induce diabetes (Diabetic group). Diabetes was controlled with insulin in the Insulin-treated group. Female rats in the control group received normal saline instead of STZ. Male newborns were euthanized at P0, P7, and P14, and the expression of α2-adrenergic and M2 muscarinic receptors in the primary visual cortex was determined using immunohistochemistry (IHC).
The study showed that α2-adrenergic and M2 muscarinic receptors were significantly suppressed in all layers of the primary visual cortex of male neonates born to diabetic rats at P0, P7, and P14 compared to the control group. The highest expression was for the Con group at P14 and the lowest one was in the Dia group at P0 for both receptors. The insulin treatment in diabetic mothers modulated the expression of these receptors to normal levels in their newborns.
The results demonstrate maternal diabetes decreases the expression of α2-adrenergic and M2 muscarinic receptors in the primary visual cortex of male offspring born to diabetic rats. Insulin treatment can offset these effects of diabetes.
本研究旨在探讨母体糖尿病对雄性仔鼠大脑初级视皮层α2-肾上腺素能和 M2 毒蕈碱受体表达的影响。
成年雌性大鼠单次腹腔注射链脲佐菌素（STZ）诱导糖尿病（糖尿病组）。胰岛素治疗组用胰岛素控制糖尿病。对照组大鼠给予生理盐水代替 STZ。雄性仔鼠于 P0、P7 和 P14 处死，采用免疫组织化学法（IHC）检测大脑初级视皮层α2-肾上腺素能和 M2 毒蕈碱受体的表达。
研究表明，与对照组相比，糖尿病大鼠仔鼠大脑初级视皮层各层α2-肾上腺素能和 M2 毒蕈碱受体在 P0、P7 和 P14 时均显著下调。两种受体在 P14 时 Con 组表达最高，在 P0 时 Dia 组表达最低。糖尿病母亲的胰岛素治疗可使新生儿这些受体的表达调节至正常水平。
研究结果表明，母体糖尿病可降低雄性仔鼠大脑初级视皮层α2-肾上腺素能和 M2 毒蕈碱受体的表达，胰岛素治疗可逆转糖尿病的这些影响。
