Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Regenerative Medicine, Organ Procurment and Disciplinary Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Neurosci Lett. 2022 Sep 14;787:136820. doi: 10.1016/j.neulet.2022.136820. Epub 2022 Jul 30.
Despite the high prevalence of diabetes in the world, its possible effects throughut pregnancy on neonatal auditory nervous system development are still unknown. In the present research, maternal diabetes' impact on the M2 and Adrenergic receptors expression in the inferior colliculus (IC) of male newborn rats was investigated.
Female rats were grouped into three: sham, insulin-treated diabetic, and diabetic. Diabetes was induced through streptozotocin (STZ) injection as one dose intraperitoneally (65 mg/kg). After mating and delivery, male rats were euthanized on P0, P7, and P14. Immunohistochemistry (IHC) was used to study the distribution pattern of receptors.
The present study indicated that the expression of M2 receptors in the diabetic group was significantly increased in pairwise comparisons in the sham and diabetic treated with insulin groups (P < 0.001, each). The highest M2 expression was for the diabetic group on P14 and the lowest one was for the sham group on P0. The Adrenergic receptors expression in the diabetic group was significantly reduced in pairwise comparisons in the sham and diabetic treated with insulin groups (P < 0.001, each). The highest Adrenergic expression was for the sham group on P14 and the lowest one was for the diabetic group on P0. There was no significant difference between the sham and insulin groups regarding all receptors expression.
This study demonstrated a time-dependent significant decrease in Adrenergic but a time-dependent significant increase in M2 receptors expression.
尽管糖尿病在全球的患病率很高，但它在妊娠期间对新生儿听觉神经系统发育的潜在影响尚不清楚。本研究旨在探讨母体糖尿病对雄性新生大鼠下丘脑中 M2 和肾上腺素能受体表达的影响。
将雌性大鼠分为三组：假手术组、胰岛素治疗糖尿病组和糖尿病组。糖尿病通过单次腹腔注射链脲佐菌素（STZ）（65mg/kg）诱导。交配和分娩后，雄性大鼠于 P0、P7 和 P14 处死。免疫组织化学（IHC）用于研究受体的分布模式。
本研究表明，与假手术组和胰岛素治疗糖尿病组相比，糖尿病组 M2 受体的表达在 P0 时显著增加（P<0.001，每组）。糖尿病组在 P14 时 M2 受体表达最高，假手术组在 P0 时表达最低。与假手术组和胰岛素治疗糖尿病组相比，糖尿病组 Adrenergic 受体的表达在 P0 时显著降低（P<0.001，每组）。Adrenergic 受体表达在 P14 时最高，在 P0 时最低。与假手术组相比，胰岛素治疗糖尿病组所有受体的表达均无显著差异。
本研究表明，Adrenergic 受体表达呈时间依赖性显著降低，而 M2 受体表达呈时间依赖性显著增加。
