School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Shaanxi Research Institute of Agricultural Products Processing Technology, Xi'an 710021, China.
School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China.
Food Res Int. 2021 Dec;150(Pt A):110721. doi: 10.1016/j.foodres.2021.110721. Epub 2021 Sep 27.
The current study applied an untargeted metabolomics approach by ultra high performance liquid chromatography quadrupole-orbitaltrap high resolution mass spectrometry (UHPLC-Q-Oritrap-MS) to identify the chemical composition of irradiated goat meat and investigate the effect of irradiation on its metabolic profile and meat quality. A total of 103 metabolites were identified as differential metabolites responsible for metabolic changes in irradiated goat meat, which were involved in phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism and purine metabolism. Differential metabolites comprising amino acids, nucleotides and their derivatives were determined as the discriminating factors responsible for the meat quality during irradiation. Specifically, the levels of L-phenylalanine, L-isoleucine, L-histidine, guanosine, guanine, creatinine, glutathione and nicotinic acid were increased while inosine 5'-monophosphate (IMP) and guanosine 5'-monophosphate (GMP) were decreased. Overall, except for L-phenylalanine and guanine, other related metabolites significantly decreased with storage. This study contributes to a comprehensive understanding of the effect of irradiation doses and storage time on goat meat metabolism at the molecular level, so as to assess the quality of irradiated goat meat. Satisfactory results with linearity (R > 0.995), precision (RSD less than 8.9%) and recovery (83%-106%) were obtained, demonstrating that the untargeted mebabolomics approach was appropriate for monitoring the changes of small molecular metabolites in irradiated goat meat and irradiation is a feasible method for goat meat preservation.
本研究采用超高效液相色谱-四极杆-轨道阱高分辨质谱（UHPLC-Q-Orbitrap-MS）的非靶向代谢组学方法，鉴定辐照羊肉的化学成分，研究辐照对其代谢谱和肉质的影响。共鉴定出 103 种差异代谢物，这些差异代谢物与辐照羊肉的代谢变化有关，涉及苯丙氨酸、酪氨酸和色氨酸生物合成、苯丙氨酸代谢和嘌呤代谢。包含氨基酸、核苷酸及其衍生物的差异代谢物被确定为辐照过程中导致肉质变化的鉴别因子。具体来说，L-苯丙氨酸、L-异亮氨酸、L-组氨酸、鸟苷、鸟嘌呤、肌酸、谷胱甘肽和烟酸的水平升高，而肌苷 5'-单磷酸（IMP）和鸟苷 5'-单磷酸（GMP）的水平降低。总体而言，除了 L-苯丙氨酸和鸟嘌呤之外，其他相关代谢物在储存过程中显著减少。本研究有助于全面了解辐照剂量和储存时间对羊肉代谢的分子水平影响，从而评估辐照羊肉的质量。该方法具有良好的线性（R>0.995）、精密度（RSD 小于 8.9%）和回收率（83%-106%），表明非靶向代谢组学方法适用于监测辐照羊肉中小分子代谢物的变化，辐照是羊肉保鲜的一种可行方法。
