Wang Min, Zhao Luyao, Niu Yu, Qin Shu, Zhang Lixin, Jia Liyan, Jing Xu
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Graduate Education Innovation Center on Baijiu Bioengineering in Shanxi Province, Taigu, Shanxi 030801, China.
Food Chem X. 2023 May 10;18:100711. doi: 10.1016/j.fochx.2023.100711. eCollection 2023 Jun 30.
In this study, a magnetic deep eutectic solvent coupled with dispersive liquid-liquid microextraction using high-performance liquid chromatography (MDES-DLLME-HPLC) was developed to detect strobilurin fungicides. The green hydrophobic MDES synthesized by methyltrioctylammonium chloride, ferric chloride, and heptanoic acid was used as an extraction solvent, which was dispersed by vortex and separated by an external magnetic field. The use of toxic solvents was avoided, and the separation time was reduced. The best experimental results were obtained through single factor and response surface optimization. The method had a good linear relationship with R > 0.996. The limit of detection (LOD) ranged from 0.001 to 0.002 mg L. The extraction recoveries were 81.9-108.9%. The proposed method was rapid and green, and it has been successfully applied to detection of strobilurin fungicides in water, juice, and vinegar.
在本研究中，开发了一种结合磁性深共熔溶剂与分散液液微萃取的高效液相色谱法（MDES-DLLME-HPLC）来检测甲氧基丙烯酸酯类杀菌剂。由氯化甲基三辛基铵、氯化铁和庚酸合成的绿色疏水性MDES用作萃取溶剂，通过涡旋分散并利用外部磁场进行分离。该方法避免了使用有毒溶剂，并缩短了分离时间。通过单因素和响应面优化获得了最佳实验结果。该方法具有良好的线性关系，R>0.996。检测限（LOD）为0.001至0.002 mg/L。萃取回收率为81.9-108.9%。所提出的方法快速且环保，已成功应用于水、果汁和醋中甲氧基丙烯酸酯类杀菌剂的检测。
