College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China; Graduate Education Innovation Center on Baijiu Bioengineering in Shanxi Province, Taigu, Shanxi 030801, PR China.
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
Food Chem. 2020 Jul 1;317:126424. doi: 10.1016/j.foodchem.2020.126424. Epub 2020 Feb 17.
In this study, a simple, efficient, and green effervescence tablet-assisted microextraction method based on the solidification of deep eutectic solvent (ETA-ME-SDES) was developed to determine picoxystrobin, pyraclostrobin, and trifloxystrobin in water, juice, wine, and vinegar samples by HPLC. An eco-friendly, hydrophobic, deep eutectic solvent (DES, acting as the extraction solvent) was synthesized by thymol and octanoic acid in the molar ratio of 1:5. The extraction solvent dispersed in sample solutions with the assistance of pH adjustment and effervescence reaction, and was collected after solidification in an ice bath. Several essential conditions, including the type and the volume of DESs, the amount of ammonia hydroxide, and the components of effervescence tablets were optimized. The limits of detection ranged from 0.15 to 0.38 μg L. Extraction recovery ranged from 77.4 to 106.9%. The proposed method was successful in determining the amount of strobilurin fungicides in water, juice, wine, and vinegar samples.
在这项研究中，开发了一种简单、高效、绿色的基于深共晶溶剂固化的冒泡片剂辅助微萃取方法（ETA-ME-SDES），通过 HPLC 测定水样、果汁、葡萄酒和醋样中的吡唑醚菌酯、咯菌腈和三氟醚菌唑。通过百里酚和辛酸以 1:5 的摩尔比合成了一种环保、疏水性的深共晶溶剂（DES，用作萃取溶剂）。在 pH 调节和冒泡反应的帮助下，萃取溶剂分散在样品溶液中，并在冰浴中凝固后收集。优化了几个重要条件，包括 DES 的类型和体积、氨水量以及冒泡片剂的成分。检测限范围为 0.15 至 0.38μg/L。萃取回收率范围为 77.4%至 106.9%。该方法成功地测定了水样、果汁、葡萄酒和醋样中啶氧菌酯类杀菌剂的含量。
