人参皂苷Rg1调节秀丽隐杆线虫的脂质代谢和温度适应性。
Ginsenosides Rg1 regulate lipid metabolism and temperature adaptation in Caenorhabditis elegans.
作者信息
Shi Hao, Zhao Jiamin, Li Yiwen, Li Junjie, Li Yunjia, Zhang Jia, Qiu Zhantu, Wu Chaofeng, Qin Mengchen, Liu Chang, Zeng Zhiyun, Zhang Chao, Gao Lei
机构信息
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China.
Department of Biochemistry, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.
出版信息
J Ginseng Res. 2023 Jul;47(4):524-533. doi: 10.1016/j.jgr.2022.11.005. Epub 2022 Nov 17.
BACKGROUND
Obesity is a risk factor for aging and many diseases, and the disorder of lipid metabolism makes it prominent. This study aims to investigate the effect of ginsenoside Rg1 on aging, lipid metabolism and stress resistance.
METHODS
Rg1 was administered to () cultured in NGM or GNGM. The lifespan, locomotory activity, lipid accumulation, cold and heat stress resistance and related mRNA expression of the worms were examined. Gene knockout mutants were used to clarify the effect on lipid metabolism of Rg1. GFP-binding mutants were used to observe the changes in protein expression.
RESULTS
We reported that Rg1 reduced lipid accumulation and improved stress resistance in . Rg1 significantly reduced the expression of fatty acid synthesis-related genes and lipid metabolism-related genes in . However, Rg1 did not affect the fat storage in double mutant or mutant. Combined with network pharmacology, we clarified the possible pathways and targets of Rg1 in lipid metabolism. In addition, Rg1-treated showed a higher expression of anti-oxidative genes and heat shock proteins, which might contribute to stress resistance.
CONCLUSION
Rg1 reduced fat accumulation by regulating lipid metabolism via and enhanced stress resistance by its antioxidant effect in .
背景
肥胖是衰老和许多疾病的危险因素，脂质代谢紊乱使其更为突出。本研究旨在探讨人参皂苷Rg1对衰老、脂质代谢和抗应激能力的影响。
方法
将Rg1施用于在NGM或GNGM中培养的（）。检测线虫的寿命、运动活性、脂质积累、抗寒和耐热应激能力以及相关mRNA表达。使用基因敲除突变体来阐明Rg1对脂质代谢的影响。使用绿色荧光蛋白结合突变体来观察蛋白质表达的变化。
结果
我们报道Rg1减少了（）中的脂质积累并提高了抗应激能力。Rg1显著降低了（）中脂肪酸合成相关基因和脂质代谢相关基因的表达。然而，Rg1对（）双突变体或（）突变体中的脂肪储存没有影响。结合网络药理学，我们阐明了Rg1在脂质代谢中的可能途径和靶点。此外，经Rg1处理的（）显示出抗氧化基因和热休克蛋白的表达较高，这可能有助于抗应激能力。
结论
Rg1通过（）调节脂质代谢减少脂肪积累，并通过其抗氧化作用增强（）的抗应激能力。
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