Department of Biotechnology, Meerut Institute of Engineering & Technology, Meerut, 250005, India.
NCoE-SAM, Department of Pediatrics, KSCH, Lady Hardinge Medical College, New Delhi, 110001, India.
Sci Rep. 2023 Jul 3;13(1):10731. doi: 10.1038/s41598-023-37602-2.
The present investigation was conducted to assess the nutritional diverseness and identify novel genetic resources to be utilized in chickpea breeding for macro and micro nutrients. The plants were grown in randomized block design. Nutritional and phytochemical properties of nine chickpea genotypes were estimated. The EST sequences from NCBI database were downloaded in FASTA format, clustered into contigs using CAP3, mined for novel SSRs using TROLL analysis and primer pairs were designed using Primer 3 software. Jaccard's similarity coefficients were used to compare the nutritional and molecular indexes followed by dendrograms construction employing UPGMA approach. The genotypes PUSA-1103, K-850, PUSA-1108, PUSA-1053 and the EST-SSR markers including the 5 newly designed namely ICCeM0012, ICCeM0049, ICCeM0067, ICCeM0070, ICCeM0078, SVP55, SVP95, SVP96, SVP146, and SVP217 were found as potential donor/marker resources for the macro-micro nutrients. The genotypes differed (p < 0.05) for nutritional properties. Amongst newly designed primers, 6 were found polymorphic with median PIC (0.46). The alleles per primer ranged 1 to 8. Cluster analysis based on nutritional and molecular diversities partially matched to each other in principle. The identified novel genetic resources may be used to widen the germplasm base, prepare maintainable catalogue and identify systematic blueprints for future chickpea breeding strategies targeting macro-micro nutrients.
本研究旨在评估营养多样性，并确定可用于鹰嘴豆宏量和微量营养的新型遗传资源。采用随机区组设计种植植物。评估了 9 个鹰嘴豆基因型的营养和植物化学特性。从 NCBI 数据库下载的 EST 序列以 FASTA 格式下载，使用 CAP3 聚类为 contigs，使用 TROLL 分析挖掘新的 SSR，并使用 Primer 3 软件设计引物。使用 Jaccard 相似系数比较营养和分子指标，然后使用 UPGMA 方法构建聚类树。基因型 PUSA-1103、K-850、PUSA-1108、PUSA-1053 和 EST-SSR 标记，包括新设计的 5 个标记 ICCeM0012、ICCeM0049、ICCeM0067、ICCeM0070、ICCeM0078、SVP55、SVP95、SVP96、SVP146 和 SVP217，被认为是宏量-微量营养素的潜在供体/标记资源。基因型在营养特性上存在差异（p < 0.05）。在新设计的引物中，有 6 个表现出多态性，中位 PIC（0.46）。每个引物的等位基因数为 1 至 8。基于营养和分子多样性的聚类分析在原则上彼此部分匹配。鉴定出的新型遗传资源可用于拓宽种质基础，编制可持续性目录，并为未来针对宏量和微量营养的鹰嘴豆育种策略确定系统蓝图。
