Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China.
Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China.
Biomater Sci. 2023 Aug 8;11(16):5439-5461. doi: 10.1039/d3bm00724c.
Collagen, classically derived from animal tissue, is an all-important protein material widely used in biomedical materials, cosmetics, fodder, food, . The production of recombinant collagen through different biological expression systems using bioengineering techniques has attracted significant interest in consideration of increasing market demand and the process complexity of extraction. Green biomanufacturing of recombinant collagen has become one of the focus topics. While the bioproduction of recombinant collagens (type I, II, III, .) has been commercialized in recent years, the biosynthesis of recombinant collagen is extremely challenging due to protein immunogenicity, yield, degradation, and other issues. The rapid development of synthetic biology allows us to perform a heterologous expression of proteins in diverse expression systems, thus optimizing the production and bioactivities of recombinant collagen. This review describes the research progress in the bioproduction of recombinant collagen over the past two decades, focusing on different expression systems (prokaryotic organisms, yeasts, plants, insects, mammalian and human cells, ). We also discuss the challenges and future trends in developing market-competitive recombinant collagens.
胶原蛋白来源于动物组织，是一种非常重要的蛋白质材料，广泛应用于生物医学材料、化妆品、饲料、食品等领域。考虑到市场需求的增加和提取过程的复杂性，通过不同的生物表达系统利用生物工程技术生产重组胶原蛋白引起了广泛关注。绿色生物制造重组胶原蛋白已成为研究热点之一。近年来，重组胶原蛋白（I 型、II 型、III 型等）的生物生产已经商业化，但由于蛋白质的免疫原性、产量、降解等问题，重组胶原蛋白的生物合成极具挑战性。合成生物学的快速发展使我们能够在不同的表达系统中进行异源蛋白表达，从而优化重组胶原蛋白的生产和生物活性。本文综述了过去 20 年来在重组胶原蛋白生物生产方面的研究进展，重点介绍了不同的表达系统（原核生物、酵母、植物、昆虫、哺乳动物和人细胞等）。我们还讨论了开发具有市场竞争力的重组胶原蛋白所面临的挑战和未来趋势。
