Shen Yanjie, Yang Guiqiang, Miao Xuexia, Shi Zhenying
Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
University of Chinese Academy of Sciences, Shanghai, 200032, China.
Rice (N Y). 2023 Jul 4;16(1):30. doi: 10.1186/s12284-023-00646-z.
Brown planthopper (BPH) is the most destructive insect pest to rice that causes tremendous yield loss each year in rice planting Asia and South-East Asia areas. Compared with traditional chemical-based treatment, utilization of plant endogenous resistance is a more effective and environmental-friendly way for BPH control. Accordingly, quite a few quantitative trait loci (QTLs) for BPH resistance were cloned using forward genetics. However, BPH is apt to change quickly into new biotypes to overcome plant resistance, therefore, new resistance resources and genes are continuously needed. miRNAs are important regulators in both plant development and physiological regulation including immunity, and might be used as effective supplements for BPH resistance QTLs. miR159 is an ancient and conserved miRNA. In this study, we found that each OsMIR159 gene in rice responded to BPH feeding very obviously, and genetic function assay proved them to negatively regulate BPH resistance, with STTM159 showing resistance to BPH, and over expression of OsmiR159d susceptible to BPH. One target genes of OsmiR159, OsGAMYBL2, positively regulated BPH resistance. Further biochemical studies revealed that OsGAMYBL2 could directly bind to the promoter of G-protein γ subunit encoding GS3 gene and repress its expression. And genetically, GS3 responded to BPH feeding promptly and negatively regulated BPH resistance, GS3 over expression plants were susceptible to BPH, while GS3 knock-out plants were resistant to BPH. Thus, we identified new function of OsmiR159-OsGAMYBL2 in mediating BPH response, and revealed a new OsmiR159-G protein pathway that mediates BPH resistance in rice.
褐飞虱是对水稻危害最大的害虫，每年在亚洲和东南亚水稻种植区都会造成巨大的产量损失。与传统的化学防治方法相比，利用植物内源抗性是一种更有效且环保的褐飞虱防治方法。因此，通过正向遗传学克隆了不少抗褐飞虱的数量性状基因座（QTL）。然而，褐飞虱很容易迅速演变成新的生物型以克服植物抗性，因此，持续需要新的抗性资源和基因。miRNA是植物发育和包括免疫在内的生理调节中的重要调节因子，可能作为抗褐飞虱QTL的有效补充。miR159是一种古老且保守的miRNA。在本研究中，我们发现水稻中的每个OsMIR159基因对褐飞虱取食反应都非常明显，遗传功能分析证明它们对褐飞虱抗性起负调控作用，STTM159表现出对褐飞虱的抗性，而OsmiR159d过表达则对褐飞虱敏感。OsmiR159的一个靶基因OsGAMYBL2对褐飞虱抗性起正调控作用。进一步的生化研究表明，OsGAMYBL2可以直接结合编码G蛋白γ亚基的GS3基因的启动子并抑制其表达。在遗传学上，GS3对褐飞虱取食反应迅速，对褐飞虱抗性起负调控作用，GS3过表达植株对褐飞虱敏感，而GS3敲除植株对褐飞虱具有抗性。因此，我们鉴定了OsmiR159 - OsGAMYBL2在介导褐飞虱反应中的新功能，并揭示了一条介导水稻褐飞虱抗性的新的OsmiR159 - G蛋白途径。
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