Le Xuyen Thi Kim, Do Roan Thi, Doan Huong Thi Thanh, Nguyen Khue Thi, Pham Linh Thi Khanh, Le Thanh Hoa
Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam.
Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam.
Arch Virol. 2023 Jul 4;168(8):201. doi: 10.1007/s00705-023-05830-6.
Since 1987, infectious bursal disease virus (IBDV) has circulated and evolved in Vietnam, but little is known about the genotypes present. IBDV samples were collected in 1987, 2001-2006, 2008, 2011, 2015-2019, and 2021 in 18 provinces. We conducted phylogenotyping analysis based on an alignment of 143 VP2-HVR (hypervariable region) sequences from 64 Vietnamese isolates (26 previous and 38 additional sequences and two vaccines, and alignment of 82 VP1 B-marker sequences, including one vaccine and four Vietnamese field strains. The analysis identified three A-genotypes, A1, A3, and A7, and two B-genotypes, B1 and B3, among the Vietnamese IBDV isolates. The lowest average evolutionary distance (8.6%) was seen between the A1 and A3 genotypes, and the highest (21.7%) was between A5 and A7, while there was a distance of 14% between B1 and B3 and 17% between B3 and B2. Unique signature residues were observed for genotypes A2, A3, A5, A6, and A8, which could be used for genotypic discrimination. A timeline statistical summary revealed that the A3-genotype predominated (79.8% presence) in Vietnam from 1987 to 2021 and that it remained the dominant IBDV genotype over the last five years (2016-2021). The current study contributes to a better understanding of the circulating genotypes and evolution of IBDV in Vietnam and worldwide.
自1987年以来，传染性法氏囊病病毒（IBDV）在越南传播并进化，但对当地存在的基因型了解甚少。于1987年、2001 - 2006年、2008年、2011年、2015 - 2019年及2021年在越南18个省份采集了IBDV样本。我们基于64株越南分离株（26株先前序列和38株新增序列以及两种疫苗）的143个VP2 - HVR（高变区）序列比对，以及82个VP1 B标记序列（包括一种疫苗和四株越南田间毒株）的比对进行系统发育分型分析。分析在越南IBDV分离株中鉴定出三种A基因型，即A1、A3和A7，以及两种B基因型，即B1和B3。A1和A3基因型之间的平均进化距离最低（8.6%），A5和A7之间最高（21.7%），而B1和B3之间的距离为14%，B3和B2之间为17%。观察到A2、A3、A5、A6和A8基因型的独特特征性残基，可用于基因型鉴别。时间线统计总结显示，1987年至2021年期间A3基因型在越南占主导地位（出现率为79.8%），并且在过去五年（2016 - 2021年）仍是主要的IBDV基因型。本研究有助于更好地了解越南及全球范围内IBDV的流行基因型及其进化情况。
