Bajda Leonardo, Amaro María Marcela, Bongiovanni Guillermina A
PROBIEN (Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas), CONICET - Universidad Nacional del Comahue, CCT-Patagonia Confluencia.
PROBIEN (Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas, CONICET-Universidad Nacional del Comahue.
Rev Fac Cien Med Univ Nac Cordoba. 2023 Jun 30;80(2):99-105. doi: 10.31053/1853.0605.v80.n2.39593.
Cannabis sativa is a plant species with numerous active principles, so the list of its therapeutic uses is expanding. In this sense, there are numerous evidences of the possible medicinal use of terpenes, as well as their synergism with cannabinoids (entourage effect). Thus, as more countries contemplate the legalization and authorization of medical cannabis, the number of cannabis extraction and analysis laboratories is increasing to meet the demand, requiring adequate analytical tools.
In response to numerous inquiries from physicians, analytical laboratories and users, the PROBIEN chromatography laboratory has selected two methods for the analysis of terpenes in Cannabis oil by gas chromatography technique (GC-FID). The methods are described using HP-5 and Innowax columns. The external standard method was used for the quantitative determination of β-Pinene, Myrcene, p-Cymene, Limonene, Linalool, α-Terpineol, Nerol and Geraniol.
good peak separation and reproducibility were observed, appropriate for the identification and quantification of the main terpenes in Cannabis extracts. The area/concentration ratio was linear in the range of 0.0005 to 2.0 mg/ml.
The described methods allow the identification and quantification of the major terpenes in Cannabis oil for an adequate quality control.
大麻是一种含有多种活性成分的植物物种，因此其治疗用途的清单正在不断扩大。从这个意义上说，有大量证据表明萜类化合物可能具有药用价值，以及它们与大麻素的协同作用（伴随效应）。因此，随着越来越多的国家考虑将医用大麻合法化和授权使用，大麻提取和分析实验室的数量不断增加以满足需求，这就需要合适的分析工具。
为回应医生、分析实验室和用户的众多询问，PROBIEN色谱实验室选择了两种通过气相色谱技术（GC-FID）分析大麻油中萜类化合物的方法。使用HP-5柱和Innowax柱对方法进行了描述。采用外标法对β-蒎烯、月桂烯、对伞花烃、柠檬烯、芳樟醇、α-松油醇、橙花醇和香叶醇进行定量测定。
观察到良好的峰分离和重现性，适用于大麻提取物中主要萜类化合物的鉴定和定量。面积/浓度比在0.0005至2.0 mg/ml范围内呈线性。
所描述的方法能够对大麻油中的主要萜类化合物进行鉴定和定量，以实现充分的质量控制。
