Kubo K, Takagi T
Anal Biochem. 1986 Jul;156(1):11-6. doi: 10.1016/0003-2697(86)90146-6.
Although polyacrylamide gel has no affinity to lithium dodecyl sulfate (LDS) at 25 degrees C, the gel maximally binds 17 mg of LDS per gram dry weight at 4 degrees C. When polyacrylamide gel electrophoresis is carried out at 4 degrees C in the presence of LDS instead of sodium dodecyl sulfate (SDS) using a continuous buffer system, migration of proteins with lower molecular weight is accelerated as a result of the deficiency of LDS in the frontal region of the gel. When the gel is saturated with LDS, electrophoresis in the presence of LDS at 4 degrees C shows a resolution higher than that of SDS-polyacrylamide gel electrophoresis at 25 degrees C.
尽管在25℃时聚丙烯酰胺凝胶与十二烷基硫酸锂（LDS）没有亲和力，但在4℃时，该凝胶每克干重最多可结合17毫克LDS。当在连续缓冲系统中于4℃、存在LDS而非十二烷基硫酸钠（SDS）的情况下进行聚丙烯酰胺凝胶电泳时，由于凝胶前沿区域LDS的缺乏，分子量较低的蛋白质迁移速度加快。当凝胶用LDS饱和后，在4℃、存在LDS的情况下进行电泳，其分辨率高于在25℃时的SDS-聚丙烯酰胺凝胶电泳。
