低pH值和低温条件下的十二烷基硫酸盐/聚丙烯酰胺凝胶电泳
Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
作者信息
Lichtner R, Wolf H U
出版信息
Biochem J. 1979 Sep 1;181(3):759-61. doi: 10.1042/bj1810759.
Abstract
A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.
摘要
本文描述了一种用于对pH值和温度敏感的生物中间体进行十二烷基硫酸盐/聚丙烯酰胺凝胶电泳的简单方法。该方法基于一种在2-4摄氏度的温度和2-4的pH值下工作的催化剂体系，以及一种含有Li+或Tris [CH2OH--C(CH2OH)2--NH3+]而非Na+的合适缓冲体系。该体系不会导致1%十二烷基硫酸盐沉淀。
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