Safaeian Parya, Yazdian Fatemeh, Khosravi-Darani Kianoush, Rashedi Hamid, Lackner Maximilian
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Department of Food Technology Research, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Front Bioeng Biotechnol. 2023 Jun 19;11:1137749. doi: 10.3389/fbioe.2023.1137749. eCollection 2023.
P3HB (poly-β-hydroxybutyrate), an energy-storage compound of several microorganisms, can be used as bioplastics material. P3HB is completely biodegradable under aerobic and aerobic conditions, also in the marine environment. The intracellular agglomeration of P3HB was examined employing a methanotrophic consortium. Supplanting fossil, non-degradable polymers by P3HB can significantly reduce the environmental impact of plastics. Utilizing inexpensive carbon sources like CH (natural gas, biogas) is a fundamental methodology to make P3HB production less costly, and to avoid the use of primary agricultural products such as sugar or starch. Biomass growth in polyhydroxyalkanoates (PHA) in general and in Poly (3-hydroxybutyrate) manufacture in specific could be a foremost point, so here the authors focus on natural gas as a proper carbon source and on the selection of bioreactors to produceP3HB, and in future further PHA, from that substrate. CH can also be obtained from biomass, e.g., biogas, syngas methanation or power-to-gas (synthetic natural gas, SNG). Simulation software can be utilized for examination, optimizing and scale-up of the process as shown in this paper. The fermentation systems continuously stirred tank reactor (CSTR), forced-liquid vertical loop bioreactor (VTLB), forced-liquid horizontal tubular loop bioreactor (HTLB), airlift (AL) fermenter and bubble column (BC) fermenter were compared for their methane conversion, kLa value, productivity, advantages and disadvantages. Methane is compared to methanol and other feedstocks. It was discovered that under optimum processing circumstances and using , the cells accumulated 51.6% cell dry mass of P3HB in the VTLB setup.
聚-β-羟基丁酸酯（P3HB）是多种微生物的储能化合物，可用作生物塑料材料。P3HB在有氧和无氧条件下均可完全生物降解，在海洋环境中也是如此。采用甲烷营养菌聚生体研究了P3HB的细胞内团聚情况。用P3HB替代化石类不可降解聚合物可显著降低塑料对环境的影响。利用天然气、沼气等廉价碳源是降低P3HB生产成本并避免使用糖或淀粉等初级农产品的基本方法。一般而言，聚羟基脂肪酸酯（PHA）中的生物质生长，特别是聚（3-羟基丁酸酯）生产中的生物质生长可能是一个首要问题，因此本文作者重点关注天然气作为合适的碳源以及用于生产P3HB（未来还可生产其他PHA）的生物反应器的选择。天然气也可从生物质中获得，例如沼气、合成气甲烷化或电力制气（合成天然气，SNG）。如本文所示，可利用模拟软件对该过程进行检测、优化和放大。对连续搅拌罐式反应器（CSTR）、强制液体垂直环流生物反应器（VTLB）、强制液体水平管式环流生物反应器（HTLB）、气升式（AL）发酵罐和鼓泡塔（BC）发酵罐等发酵系统的甲烷转化率、体积传质系数kLa值、生产率、优缺点进行了比较。将甲烷与甲醇及其他原料进行了比较。结果发现，在最佳工艺条件下，使用[具体条件未给出]时，细胞在VTLB装置中积累了占细胞干重51.6%的P3HB。
