Patel Sanjay K S, Singh Deepshikha, Pant Diksha, Gupta Rahul K, Busi Siddhardha, Singh Rahul V, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Department of Biotechnology, Hemvati Nandan Bahuguna Garhwal University (A Central University), Srinagar 246174, Uttarakhand, India.
Polymers (Basel). 2024 Sep 11;16(18):2570. doi: 10.3390/polym16182570.
Methanotrophs are bacteria that consume methane (CH) as their sole carbon and energy source. These microorganisms play a crucial role in the carbon cycle by metabolizing CH (the greenhouse gas), into cellular biomass and carbon dioxide (CO). Polyhydroxyalkanoates (PHAs) are biopolymers produced by various microorganisms, including methanotrophs. PHA production using methanotrophs is a promising strategy to address growing concerns regarding plastic pollution and the need for sustainable, biodegradable materials. Various factors, including nutrient availability, environmental conditions, and metabolic engineering strategies, influence methanotrophic production. Nutrient limitations, particularly those of nitrogen or phosphorus, enhance PHA production by methanotrophs. Metabolic engineering approaches, such as the overexpression of key enzymes involved in PHA biosynthesis or the disruption of competing pathways, can also enhance PHA yields by methanotrophs. Overall, PHA production by methanotrophs represents a sustainable and versatile approach for developing biomedical materials with numerous potential applications. Additionally, alternative feedstocks, such as industrial waste streams or byproducts can be explored to improve the economic feasibility of PHA production. This review briefly describes the potential of methanotrophs to produce PHAs, with recent updates and perspectives.
甲烷营养菌是一类以甲烷（CH）作为唯一碳源和能源的细菌。这些微生物通过将CH（一种温室气体）代谢为细胞生物质和二氧化碳（CO），在碳循环中发挥着关键作用。聚羟基脂肪酸酯（PHA）是包括甲烷营养菌在内的多种微生物产生的生物聚合物。利用甲烷营养菌生产PHA是一种很有前景的策略，可应对人们对塑料污染日益增长的担忧以及对可持续、可生物降解材料的需求。包括养分可用性、环境条件和代谢工程策略在内的各种因素都会影响甲烷营养菌的生产。养分限制，尤其是氮或磷的限制，会提高甲烷营养菌的PHA产量。代谢工程方法，如PHA生物合成中关键酶的过表达或竞争途径的破坏，也可以提高甲烷营养菌的PHA产量。总体而言，甲烷营养菌生产PHA代表了一种可持续且通用的方法，可用于开发具有众多潜在应用的生物医学材料。此外，可以探索替代原料，如工业废水流或副产品，以提高PHA生产的经济可行性。本综述简要介绍了甲烷营养菌生产PHA的潜力，并提供了最新进展和观点。
