Gerdes A M, Moore J A, Hines J M, Kirkland P A, Bishop S P
Anat Rec. 1986 Aug;215(4):420-6. doi: 10.1002/ar.1092150414.
Three independent methods were evaluated in an effort to obtain reliable values for myocyte size in adult Sprague-Dawley rats. Cell volume was determined from isolated myocytes by a Coulter Channelyzer system. Cell volume was also determined from the product of length and cross-sectional area of isolated myocytes. Additionally, myocyte cross-sectional area was measured morphometrically from electron micrographs of whole perfusion-fixed tissue. A major goal was to determine if anatomical methods used to measure cell volume produce values comparable to the more expeditious and objective Coulter Channelyzer method. The results of these experiments showed that myocyte dimensions obtained from all three techniques were similar. The second major objective was to use the above-mentioned techniques to evaluate regional differences in myocyte size. Myocyte cross-sectional area and volume were significantly larger in the endomyocardium than in the epimyocardium of the left ventricle. Right ventricle myocytes had significantly smaller volumes and cross-sectional areas than did left ventricle myocytes. There were no regional differences in cell lengths. We conclude that the Coulter Channelyzer system gives values for isolated myocyte volume that are similar to values obtained with histometric techniques; values for isolated myocyte cross-sectional area were representative of values obtained from myocytes in whole-sectioned tissue; significant regional differences in myocyte size are present in adult rat hearts; and regional variations in myocyte size are due to differences in myocyte cross-sectional area rather than cell length.
为了获得成年Sprague-Dawley大鼠心肌细胞大小的可靠值，我们评估了三种独立的方法。通过库尔特通道分析仪系统从分离的心肌细胞中测定细胞体积。细胞体积也通过分离的心肌细胞的长度和横截面积的乘积来确定。此外，从全灌注固定组织的电子显微照片中形态计量地测量心肌细胞横截面积。一个主要目标是确定用于测量细胞体积的解剖学方法所产生的值是否与更快速、客观的库尔特通道分析仪方法相当。这些实验的结果表明，从所有三种技术获得的心肌细胞尺寸相似。第二个主要目标是使用上述技术评估心肌细胞大小的区域差异。左心室内膜下心肌细胞的横截面积和体积明显大于心外膜下心肌细胞。右心室心肌细胞的体积和横截面积明显小于左心室心肌细胞。细胞长度没有区域差异。我们得出结论，库尔特通道分析仪系统给出的分离心肌细胞体积值与组织测量技术获得的值相似；分离心肌细胞横截面积的值代表从全切片组织中的心肌细胞获得的值；成年大鼠心脏中存在明显的心肌细胞大小区域差异；并且心肌细胞大小的区域差异是由于心肌细胞横截面积的差异而不是细胞长度的差异。
