Gołaszewska Agnieszka, Skawrońska Małgorzata, Niemcunowicz-Janica Anna, Pepiński Witold
Medical University of Białystok, Poland.
Arch Med Sadowej Kryminol. 2022;72(4):200-210. doi: 10.4467/16891716AMSIK.22.022.17622.
The use of new high-resolution and forensic identification capabilities for population studies offered by new multiplex methods (such as Yfiler Plus) is crucial in forensic genetics cases. The development of haplotype frequency databases is essential to take full advantage of the new Y chromosome determination capabilities.
Development of the haplotype database of the Yfiler Plus kit for a population-based sample of 534 males from northeastern Poland and calculation of suitability parameters for forensic genetics studies.
The study was conducted on a population sample of 534 unrelated males from the area of northeastern Poland using the Yfiler Plus panel of 27 markers located on the Y chromosome.
Four haplotypes appeared twice. The Discrimination Capacity (DC) of the entire set was 0.9925. The highest Gene Diversity (GD) value was calculated for DYS518 (0.86) belonging to the fast-mutation markers, while the lowest GD was calculated for DYS392 (0.42).
The results indicate the need for further research and observation of changes, both in different regions of Poland and across Europe.
新的多重方法（如Yfiler Plus）所提供的用于群体研究的高分辨率和法医鉴定新能力，在法医遗传学案件中至关重要。单倍型频率数据库的开发对于充分利用新的Y染色体检测能力至关重要。
为来自波兰东北部的534名男性的群体样本开发Yfiler Plus试剂盒的单倍型数据库，并计算法医遗传学研究的适用性参数。
使用位于Y染色体上的包含27个标记的Yfiler Plus检测板，对来自波兰东北部地区的534名无关男性的群体样本进行研究。
有四种单倍型出现了两次。整个数据集的鉴别能力（DC）为0.9925。属于快速突变标记的DYS518的基因多样性（GD）值最高（0.86），而DYS392的GD值最低（0.42）。
结果表明需要在波兰不同地区以及整个欧洲进一步开展研究并观察变化情况。
