Haddad N S, Pedersoli W M, Carson R L, Ravis W R
Am J Vet Res. 1986 Jul;47(7):1597-601.
Healthy mature cows (n = 6) were injected intrauterinally (IU) with gentamicin (50 ml of a 5% injectable solution) daily for 3 consecutive days. Venous blood and milk samples were collected at postinjection (initial) hours (PIH) 1, 3, 6, 9, 12, 24, 28, 31, 34, 37, 48, 51, 54, 57, 60, and 71, and endometrial biopsies were performed at PIH 6, 25, 48, 73, 95, and 119. Skeletal muscle biopsy samples were taken at PIH 25 and 73, and urine was collected every 1 or 2 hours during 12 consecutive hours after the first IU injection. Serum, milk, urine, and tissue concentrations of gentamicin were measured by radioimmunoassay. The highest mean serum concentration of gentamicin occurred during the 3 hours after each injection (2.49 +/- 1.46, 6.60 +/- 5.47, and 4.98 +/- 2.70 micrograms/ml). The mean peak concentration of gentamicin in milk occurred 3 to 6 hours after each injection. Mean peak urine concentration of gentamicin (256.8 +/- 127.9 micrograms/ml) was measured at PIH 6. The mean percentage of the first dose of gentamicin excreted in the urine within 12 hours was 14.78 +/- 3.56. The highest concentration of gentamicin in endometrial tissue (639.16 +/- 307.22 micrograms/g) was measured at PIH 6, decreasing to 9.64 +/- 3.55 micrograms/g before the next IU dose. Gentamicin was still detectable in endometrial tissue (0.86 +/- 0.43 microgram/g) 71 hours after the 3rd (last) IU injection.
选用6头健康成年母牛，连续3天每天经子宫内（IU）注射庆大霉素（5%可注射溶液50毫升）。在注射后（初始）1、3、6、9、12、24、28、31、34、37、48、51、54、57、60和71小时采集静脉血和乳汁样本，并在注射后6、25、48、73、95和119小时进行子宫内膜活检。在注射后25和73小时采集骨骼肌活检样本，并在首次子宫内注射后的连续12小时内每1或2小时收集一次尿液。采用放射免疫分析法测定庆大霉素的血清、乳汁、尿液和组织浓度。每次注射后的3小时内，庆大霉素的平均血清浓度最高（分别为2.49±1.46、6.60±5.47和4.98±2.70微克/毫升）。每次注射后3至6小时，乳汁中庆大霉素的平均峰值浓度出现。在注射后6小时测得庆大霉素的平均尿液峰值浓度为256.8±127.9微克/毫升。首次注射的庆大霉素在12小时内尿液排泄的平均百分比为14.78±3.56。在注射后6小时测得子宫内膜组织中庆大霉素的最高浓度为639.16±307.22微克/克，在下一次子宫内注射前降至9.64±3.55微克/克。在第3次（最后一次）子宫内注射后71小时，子宫内膜组织中仍可检测到庆大霉素（0.86±0.43微克/克）。
