Department of Orofacial Pain and Dysfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Cytokine. 2023 Sep;169:156251. doi: 10.1016/j.cyto.2023.156251. Epub 2023 Jul 3.
Dental pain is a main clinical problem in the elderly population and its assessment and treatment make special challenges for health care services. However, the age-induced alteration in dental pain perception and the underlying molecular mechanism(s) has not yet been fully clarified.
Here, the effect of aging on nociceptive behaviors following inflammatory dental pulp pain was evaluated. Since prostaglandins, nociceptive neuropeptides, and inflammatory cytokines have critical roles in the development of aging as well as pain signaling, the expression levels of COX-2, CGRP, IL-1β, IL-6, TNF-α and its converting enzyme TACE were assessed in the trigeminal ganglion of young and aged rats with dental pain. Dental pulp pain was induced by intradental application of capsaicin (100 μg). The immunofluorescence (COX-2 and CGRP) and western blot techniques were used.
The data showed that aged animals have different pattern of pain. So that, the mean of nociceptive scores was significantly greater in aged rats at 10 and 15 min after capsaicin injection. In aged rats, dental pain was persisting over 7 h, while it was disappeared at 300 min in young rats. Molecular data showed that dental pain significantly increased the expression of COX-2, CGRP, IL-1β, IL-6, TNF-α and TACE in the trigeminal ganglion of the young and aged rats. In addition, the amount of those parameters, except TACE, in capsaicin-treated aged animals were significantly (p < 0.05) greater than those in capsaicin-treated young rats.
It seems that the induction of pro-inflammatory cytokines in an acute inflammatory pulpal pain model may contribute, at least in part to the increased nociceptive behaviors and pain perception in aged rats.
牙齿疼痛是老年人群体中的一个主要临床问题，其评估和治疗给医疗保健服务带来了特殊挑战。然而，年龄对牙齿疼痛感知的影响及其潜在的分子机制尚未完全阐明。
本研究评估了炎症性牙髓疼痛后年龄对伤害性行为的影响。由于前列腺素、伤害性神经肽和炎症细胞因子在衰老和疼痛信号传导中起着关键作用，因此评估了 COX-2、CGRP、IL-1β、IL-6、TNF-α及其转化酶 TACE 在年轻和老年大鼠三叉神经节中在牙髓疼痛的表达水平。通过在牙内应用辣椒素（100μg）诱导牙髓疼痛。使用免疫荧光（COX-2 和 CGRP）和 Western blot 技术。
数据显示，老年动物表现出不同的疼痛模式。因此，在辣椒素注射后 10 和 15 分钟，老年大鼠的疼痛评分平均值显着更高。在老年大鼠中，牙齿疼痛持续超过 7 小时，而在年轻大鼠中则在 300 分钟时消失。分子数据显示，牙齿疼痛显着增加了年轻和老年大鼠三叉神经节中 COX-2、CGRP、IL-1β、IL-6、TNF-α和 TACE 的表达。此外，与辣椒素处理的年轻大鼠相比，辣椒素处理的老年动物中除 TACE 外，这些参数的量显着增加（p<0.05）。
在急性炎症性牙髓疼痛模型中诱导促炎细胞因子可能至少部分导致老年大鼠伤害性行为和疼痛感知增加。
