Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China.
Fish Shellfish Immunol. 2023 Aug;139:108927. doi: 10.1016/j.fsi.2023.108927. Epub 2023 Jul 4.
The aquaculture industry has suffered significant financial losses as a result of disease outbreaks. In particular, disease outbreaks have become a major problem that can seriously affect the sustainable development of the Macrobrachium rosenbergii aquaculture industry. It is crucial to determine the defense mechanism of the host after pathogenic invasion in order to provide effective defense measures after disease outbreaks. Shrimp, like other invertebrates, primarily depend on their innate immune systems to defend against pathogens, and recognize and resist pathogens through humoral and cellular immune responses. In this investigation, we used RNA-seq technology to investigate the transcriptome of hemocytes from M. rosenbergii induced by Staphylococcus aureus. Our main targets were immune pathways and genes related to innate immunity. RNA-seq identified 209,069 and 204,775 unigenes in the control and experimental groups, respectively. In addition, we identified 547 and 1734 differentially expressed genes (DEGs) following S. aureus challenge after 6 and 12 h (h), respectively. GO and KEGG enrichment analysis revealed that the DEGs were significantly enriched in several biological signalling pathways, including NOD-like receptor, PI3K-Akt, Toll and Imd, IL-17, TGF-beta, RIG-I-like receptor, cAMP, apoptosis, and C-type lectin receptor. Sixteen DEGs were chosen at random for qPCR verification; these results concurred with those from sequencing. Our findings revealed that immune-related genes play an important role in antibacterial activities and have specific functions for gram-positive bacteria. These results provide more data for the prevention of M. rosenbergii diseases and offer a basis for the better prevention of diseases.
水产养殖业因疾病爆发而遭受了重大的财务损失。特别是，疾病爆发已成为一个主要问题，可能会严重影响罗氏沼虾养殖业的可持续发展。确定宿主在受到病原体入侵后的防御机制至关重要，以便在疾病爆发后提供有效的防御措施。虾类与其他无脊椎动物一样，主要依赖其先天免疫系统来抵御病原体，并通过体液和细胞免疫反应来识别和抵抗病原体。在这项研究中，我们使用 RNA-seq 技术研究了罗氏沼虾血细胞在金黄色葡萄球菌诱导下的转录组。我们的主要目标是先天免疫相关的免疫途径和基因。RNA-seq 在对照组和实验组中分别鉴定出 209,069 和 204,775 个基因。此外，我们还分别鉴定出金黄色葡萄球菌感染 6 和 12 小时后（h）的 547 个和 1734 个差异表达基因（DEGs）。GO 和 KEGG 富集分析表明，DEGs 在几个生物学信号通路中显著富集，包括 NOD 样受体、PI3K-Akt、Toll 和 Imd、IL-17、TGF-β、RIG-I 样受体、cAMP、细胞凋亡和 C 型凝集素受体。随机选择了 16 个 DEGs 进行 qPCR 验证；这些结果与测序结果一致。我们的研究结果表明，免疫相关基因在抗菌活性中发挥着重要作用，并且对革兰氏阳性菌具有特定的功能。这些结果为罗氏沼虾疾病的预防提供了更多的数据，并为更好地预防疾病提供了依据。
