Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China.
Fish Shellfish Immunol. 2023 Feb;133:108533. doi: 10.1016/j.fsi.2023.108533. Epub 2023 Jan 11.
Macrobrachium rosenbergii as one of the common freshwater prawn species in Southeast Asia, which breeding industry is seriously threatened by vibriosis and causes high mortality. In this study, the RNA-seq was employed for assessing the M. rosenbergii hemocytes transcriptomes following Vibrio parahaemolyticus challenge. After challenge for 6 h (h), there were overall 1849 DEGs or differentially expressed genes, including 1542 up-regulated and 307 down-regulated genes, and there was a total of 1048 DEGs, including 510 up-regulated genes and 538 down-regulated genes, after challenge for 12 h. Mitogen-activated protein kinase (MAPK) immune-related pathways, Toll, immune deficiency (IMD), and Janus kinase (JAK)/signal transducer and activator of transcription (STAT) were among the immune pathways where a lot of the DEGs were connected. The expression patterns of 18 chosen immune-related genes were examined utilizing qRT-PCR or quantitative real-time polymerase chain reaction, which revealed that the V. parahaemolyticus infection activated the M. rosenbergii's immune response. Permutational multivariate analysis of variance (PERMANOVA) showed that V. parahaemolyticus infection modulated immune regulation and apoptosis pathways. The gathered information provided new insight into M. rosenbergii's immunity and suggested a novel approach to fight against bacterial infection.
罗氏沼虾作为东南亚地区常见的淡水虾品种之一，其养殖产业受到弧菌病的严重威胁，导致死亡率很高。在本研究中，采用 RNA-seq 技术评估了罗氏沼虾血细胞转录组在副溶血弧菌攻毒后的变化。攻毒 6 小时（h）后，共检测到 1849 个差异表达基因（DEGs），包括 1542 个上调基因和 307 个下调基因；攻毒 12 小时后，共检测到 1048 个 DEGs，包括 510 个上调基因和 538 个下调基因。丝裂原活化蛋白激酶（MAPK）免疫相关途径、Toll、免疫缺陷（IMD）和 Janus 激酶（JAK）/信号转导和转录激活因子（STAT）是与大量 DEGs 相关的免疫途径之一。利用 qRT-PCR 或定量实时聚合酶链反应（PCR）检测了 18 个选择的免疫相关基因的表达模式，结果表明副溶血弧菌感染激活了罗氏沼虾的免疫反应。PERMANOVA 分析显示，副溶血弧菌感染调节了免疫调节和细胞凋亡途径。收集到的信息为罗氏沼虾的免疫机制提供了新的见解，并为对抗细菌感染提供了新的思路。
