Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou, 313000, China.
Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou, 313000, China.
Fish Shellfish Immunol. 2023 Nov;142:109151. doi: 10.1016/j.fsi.2023.109151. Epub 2023 Oct 12.
The Macrobrachium rosenbergii industry is threatened by various Aeromonas, resulting in high mortality of adult prawns. However, there are few studies on the immune response of M. rosenbergii infected with Aeromonas dhakensis. In this study, we observed the hepatopancreas and gills histopathologically, performed a comparative transcriptome analysis of the hepatopancreas, and analyzed the candidate gene expression of immune-related genes in the hemolymph, hepatopancreas, and gills of M. rosenbergii that had been infected with A. dhakensis. Histopathology revealed the hepatopancreas was successively inflamed, followed by cellular vacuolation, lumen deformation, and finally tissue erosion; partial and severe inflammation of the gills occurred successively, and eventually the gill tissue atrophy and the gill filaments detached from the gill arch. Transcriptome analysis showed that a total of 77,742 unigenes and 8664 differentially expressed genes (DEGs), and the immune-related DEGs were mainly enriched in lysosome and phagosome pathways. In addition, 4 immune-related candidate genes (RhoA, CASP9, PKC, and DSCIGN) based on KEGG and PPI analysis were monitored at 6, 12, and 24h post injection (hpi) in hepatopancreas, hemolymph and gills. Their spatio-temporal expression results indicated that A. dhakensis have activated the immune system of M. rosenbergii. The present study may provide new information on the complex immune mechanism of M. rosenbergii.
罗氏沼虾产业受到各种气单胞菌的威胁，导致成虾死亡率高。然而，关于感染戴氏气单胞菌的罗氏沼虾免疫反应的研究较少。在本研究中，我们观察了罗氏沼虾的肝胰腺和鳃的组织病理学变化，对肝胰腺进行了比较转录组分析，并分析了感染戴氏气单胞菌后罗氏沼虾血淋巴、肝胰腺和鳃中免疫相关基因候选基因的表达。组织病理学显示肝胰腺依次发炎，随后出现细胞空泡化、管腔变形，最后组织侵蚀；鳃的部分和严重炎症依次发生，最终鳃组织萎缩，鳃丝从鳃弓上脱落。转录组分析表明，共获得 77742 条 unigenes 和 8664 条差异表达基因（DEGs），免疫相关 DEGs 主要富集在溶酶体和吞噬体途径。此外，基于 KEGG 和 PPI 分析，选择了 4 个免疫相关候选基因（RhoA、CASP9、PKC 和 DSCIGN），在肝胰腺、血淋巴和鳃中分别于注射后 6、12 和 24 小时（hpi）进行监测。它们的时空表达结果表明，戴氏气单胞菌激活了罗氏沼虾的免疫系统。本研究可能为罗氏沼虾复杂的免疫机制提供新的信息。
