School of Stomatology, Nanchang University, Nanchang 330006, China; Jiangxi Province Key Laboratory of Oral Biomedicine, Nanchang 330006, China; Jiangxi Province Clinical Research Center for Oral Disease, Nanchang 330006, China.
School of Stomatology, Nanchang University, Nanchang 330006, China; School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, China; Jiangxi Province Key Laboratory of Oral Biomedicine, Nanchang 330006, China; Jiangxi Province Clinical Research Center for Oral Disease, Nanchang 330006, China.
Int J Biol Macromol. 2023 Aug 30;247:125671. doi: 10.1016/j.ijbiomac.2023.125671. Epub 2023 Jul 3.
Insufficient bone regeneration and bacterial infection are two major concerns of bone repair materials. Poly-L-lactic acid (PLLA) have been widely used in bone tissue engineering (BTE), however, lack of osteogenic and antibacterial properties have greatly limit its clinical application. Herein, PLLA membrane was firstly treated with polydopamine (PDA), and then modified with ε-polylysine (ε-PL) and alginate (ALG) via layer-by-layer method. The (ε-PL/ALG)n composite layer coated PLLA (PLLA@(ε-PL/ALG)n) could facilitates the adhesion and osteoblast differentiation of MC3T3-E1 cells. Furthermore, PLLA@(ε-PL/ALG) presents an effective antibacterial efficacy against S. aureus and E. coli, and the bacterial survival rates of S. aureus and E. coli on PLLA@(ε-PL/ALG) were 21.5 ± 3.5 % and 13 ± 2.1 %, respectively. This work provides a promising method to design PLLA materials with osteogenic and antibacterial activity simultaneously. Furthermore, the method is also an optional choice to construct multifunctional coatings on the other substrate.
骨再生不足和细菌感染是骨修复材料的两个主要关注点。聚-L-乳酸（PLLA）已广泛应用于骨组织工程（BTE），但其成骨和抗菌性能的缺乏极大地限制了其临床应用。在此，首先用聚多巴胺（PDA）处理 PLLA 膜，然后通过层层法用 ε-聚赖氨酸（ε-PL）和藻酸盐（ALG）进行修饰。（ε-PL/ALG）n 复合层涂覆的 PLLA（PLLA@（ε-PL/ALG）n）可以促进 MC3T3-E1 细胞的黏附和成骨细胞分化。此外，PLLA@（ε-PL/ALG）对金黄色葡萄球菌和大肠杆菌具有有效的抗菌功效，金黄色葡萄球菌和大肠杆菌在 PLLA@（ε-PL/ALG）上的存活率分别为 21.5±3.5%和 13±2.1%。这项工作为设计同时具有成骨和抗菌活性的 PLLA 材料提供了一种很有前途的方法。此外，该方法也是在其他基底上构建多功能涂层的可选选择。
