Tao Ziwei, Yuan Ziyang, Zhou Dong, Qin Lang, Xiao Lan, Zhang Shihao, Liu Changsheng, Zhao Jinzhong, Li Yulin
Engineering Research Centre for Biomedical Materials of Ministry of Education, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, China.
Department of Sports Medicine, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Biomater Transl. 2023 Dec 28;4(4):280-290. doi: 10.12336/biomatertransl.2023.04.007. eCollection 2023.
Biodegradable polymer microspheres that can be used as drug carriers are of great importance in biomedical applications, however, there are still challenges in controllable preparation of microsphere surface morphology and improvement of bioactivity. In this paper, firstly, poly(L-lactic acid) (PLLA) was synthesised by ring-opening polymerisation under anhydrous anaerobic conditions and further combined with the emulsion method, biodegradable PLLA microspheres (PM) with sizes ranging from 60-100 μm and with good sphericity were prepared. In addition, to further improve the surface morphology of PLLA microspheres and enhance their bioactivity, functionalised porous PLLA microspheres loaded with magnesium oxide (MgO)/magnesium carbonate (MgCO) (PMg) were also prepared by the emulsion method. The results showed that the loading of MgO/MgCO resulted in the formation of a porous structure on the surface of the microspheres (PMg) and the dissolved Mg could be released slowly during the degradation of microspheres. In vitro cellular experiments demonstrated the good biocompatibility of PM and PMg, while the released Mg further enhanced the anti-inflammatory effect and osteogenic activity of PMg. Functionalised PMg not only show promise for controlled preparation of drug carriers, but also have translational potential for bone regeneration.
可作为药物载体的可生物降解聚合物微球在生物医学应用中具有重要意义，然而，在微球表面形态的可控制备和生物活性的提高方面仍存在挑战。本文首先在无水无氧条件下通过开环聚合合成聚（L-乳酸）（PLLA），并进一步结合乳液法，制备了尺寸范围为60-100μm且具有良好球形度的可生物降解PLLA微球（PM）。此外，为了进一步改善PLLA微球的表面形态并增强其生物活性，还通过乳液法制备了负载氧化镁（MgO）/碳酸镁（MgCO）的功能化多孔PLLA微球（PMg）。结果表明，MgO/MgCO的负载导致微球（PMg）表面形成多孔结构，并且溶解的Mg在微球降解过程中可以缓慢释放。体外细胞实验证明了PM和PMg具有良好的生物相容性，而释放的Mg进一步增强了PMg的抗炎作用和成骨活性。功能化的PMg不仅在药物载体的可控制备方面具有前景，而且在骨再生方面具有转化潜力。
