Unune A, Naviaux R K, Christian J C, Goldstein D J
Ann Clin Lab Sci. 1986 Jul-Aug;16(4):278-88.
A solid phase micro competition enzyme linked immunosorbant assay (microCELIA) was developed to measure apolipoprotein B (Apo B) in human plasma. The soluble Apo B competed with the solid phase antigen for antibody binding. After washing, alkaline phosphatase labeled goat anti-rabbit IgG antibody was added and the plates were washed and assayed. The minimal quantifiable concentration of Apo B was one mg per L. This enzyme immunoassay (EIA) yielded values which correlated with values of samples assayed in reference laboratories by radioimmunoassay (RIA) (r = 0.97) and by electroimmunoassay (r = 0.92). The electroimmunoassay overestimates the activity of hyperlipemic samples compared to microCELIA. The assay offers advantages over other existing techniques including short incubation time, high sensitivity, technical simplicity, elimination of radioisotopes, low cost, and use of a universal enzyme label.
开发了一种固相微竞争酶联免疫吸附测定法（微CELIA）来测量人血浆中的载脂蛋白B（Apo B）。可溶性Apo B与固相抗原竞争抗体结合。洗涤后，加入碱性磷酸酶标记的山羊抗兔IgG抗体，然后洗涤平板并进行测定。Apo B的最小可量化浓度为每升1毫克。这种酶免疫测定法（EIA）得出的值与参考实验室通过放射免疫测定法（RIA）（r = 0.97）和免疫电泳法（r = 0.92）测定的样品值相关。与微CELIA相比，免疫电泳法高估了高脂血症样品的活性。该测定法相对于其他现有技术具有优势，包括孵育时间短、灵敏度高、技术简单、无需使用放射性同位素、成本低以及使用通用酶标记。
