Panush R S, Somberg L B, Katz P, Longley S
Diagn Immunol. 1983;1(4):288-94.
We developed a simplified, relatively rapid, inexpensive, antigen-nonspecific, enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG)- and C3-containing circulating immune complexes (CICs), adapted from a solid-phase anti-C3 radioimmunoassay (RIA). Standards (containing purified, heat-aggregated IgG and fresh human serum) or samples were allowed to react with goat F(ab')2 antihuman C3 bound to the matrix of microtiter plates. Then alkaline phosphatase conjugated to goat IgG fraction antihuman IgG was added, followed by p-nitrophenylphosphate, optical densities determined, and concentrations of CICs calculated. We found excellent correlations between serum and plasma CIC levels by either ELISA (r = 0.95, p less than 0.01) or RIA (r = 0.89, p less than 0.01). Furthermore, ELISA quantitation of CICs correlated well with RIA (serum, n = 75, r = 0.64, p less than 0.01; plasma, n = 101, r = 0.56, p less than 0.01). By ELISA we found 32 normal subjects had 38 +/- 12 micrograms CIC/ml in serum and 34 +/- 10 micrograms CIC/ml in plasma. Patients with systemic lupus erythematosus (39% of 27 patients, p less than 0.05) had significantly elevated CIC levels compared with normal (serum, 157 +/- 50 micrograms/ml, p less than 0.01; plasma, 89 +/- 23 micrograms/ml, p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
我们开发了一种简化、相对快速、廉价、抗原非特异性的酶联免疫吸附测定（ELISA）方法，用于检测含免疫球蛋白G（IgG）和C3的循环免疫复合物（CIC），该方法改编自固相抗C3放射免疫测定（RIA）。标准品（含纯化的热聚集IgG和新鲜人血清）或样品与结合在微量滴定板基质上的山羊F(ab')2抗人C3反应。然后加入与山羊IgG组分抗人IgG偶联的碱性磷酸酶，接着加入对硝基苯磷酸酯，测定光密度，并计算CIC浓度。我们发现，通过ELISA（r = 0.95，p < 0.01）或RIA（r = 0.89，p < 0.01）检测，血清和血浆CIC水平之间具有良好的相关性。此外，ELISA对CIC的定量与RIA相关性良好（血清，n = 75，r = 0.64，p < 0.01；血浆，n = 101，r = 0.56，p < 0.01）。通过ELISA我们发现，32名正常受试者血清中CIC浓度为38±12微克/毫升，血浆中为34±10微克/毫升。与正常受试者相比，系统性红斑狼疮患者（27例中的39%，p < 0.05）的CIC水平显著升高（血清，157±50微克/毫升，p < 0.01；血浆，89±23微克/毫升，p < 0.05）。（摘要截短至250字）
