Farshadpour Fatemeh, Taherkhani Reza, Farzaneh Mohammadreza
Department of Virology, School of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, 7514633341 Iran.
Department of Pathology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
Virusdisease. 2023 Jun;34(2):257-262. doi: 10.1007/s13337-023-00819-w. Epub 2023 May 2.
Information regarding the possible carcinogenicity of human papillomaviruses (HPVs) in bladder tissue might pave the way for the prevention of bladder cancer through improving HPV vaccination of the at-risk population. To address this, this study was conducted to detect HPVs in bladder cancer tissues in the South of Iran. Bladder biopsy samples of 181 patients with bladder cancer were included in this study. The detection of HPVs was performed by nested PCR assay, targeting the L1 region of the genome, and sequencing. HPV was detected in 0.55% of the bladder cancer samples, while the non-cancerous bladder samples were negative for HPV. HPV genotype 6 was detected in this study. The HPV-positive patient was a 55-year-old man with papillary urothelial neoplasms of low malignant in stage Ta-T1. This patient was resident of Dayer city. Overall, HPV prevalence among patients with bladder cancer was not statistically associated with place of residency, gender, age, stage, and grade of the tumor (value > 0.05). The presence of HPV is extremely rare in bladder cancer biopsy specimens in the south of Iran. Therefore, the results of our study rule out the possible role of HPVs in the etiology of bladder cancer. Due to the increasing air pollution in this region and high-risk jobs, and habits such as cigarette smoking and hookah smoking, the role of these factors alongside genetic factors seems more prominent than the role of HPVs in causing bladder cancer in the south of Iran.
The online version contains supplementary material available at 10.1007/s13337-023-00819-w.
关于人乳头瘤病毒（HPV）在膀胱组织中可能的致癌性信息，或许可为通过改善高危人群的HPV疫苗接种来预防膀胱癌铺平道路。为解决这一问题，本研究旨在检测伊朗南部膀胱癌组织中的HPV。本研究纳入了181例膀胱癌患者的膀胱活检样本。通过巢式PCR检测法靶向基因组的L1区域并进行测序来检测HPV。在0.55%的膀胱癌样本中检测到HPV，而癌旁膀胱样本HPV检测为阴性。本研究中检测到HPV基因型6。HPV阳性患者为一名55岁男性，患有Ta-T1期低恶性乳头状尿路上皮肿瘤。该患者居住在代耶市。总体而言，膀胱癌患者中HPV的患病率与居住地、性别、年龄、肿瘤分期和分级无统计学关联（P值>0.05）。在伊朗南部，HPV在膀胱癌活检标本中极为罕见。因此，我们的研究结果排除了HPV在膀胱癌病因学中的可能作用。鉴于该地区空气污染加剧、高危职业以及吸烟和吸水烟等习惯，这些因素与遗传因素共同作用在伊朗南部导致膀胱癌方面似乎比HPV的作用更为突出。
在线版本包含可在10.1007/s13337-023-00819-w获取的补充材料。
