Ruditser Rebecca, Fu Xueyan, Booth Sarah L, Liu Minying, Shen Xiaohua, Shea M Kyla
United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA, United States.
Curr Dev Nutr. 2023 Jun 3;7(7):101959. doi: 10.1016/j.cdnut.2023.101959. eCollection 2023 Jul.
Enzyme-linked i2mmunosorbent assays (ELISAs) that measure circulating phylloquinone have become commercially available, but their validity is uncertain. The objective of this study was to compare plasma phylloquinone concentrations measured using two commercially available ELISAs with concentrations measured using a validated high-performance liquid chromatography (HPLC) assay in 108 samples obtained from participants in a depletion (∼10 mcg phylloquinone/d)-supplementation (∼500 mcg phylloquinone/d) study. The geometric mean of plasma phylloquinone measured with ELISA A was 0.70 nmol/L, 37% lower than that measured with HPLC. The mean of the ELISA B measures was 12.4 nmol/L, >700% higher than the HPLC measures. Plasma phylloquinone measured using HPLC was significantly lower during phylloquinone depletion than supplementation (0.4 ± 0.1 compared with 1.2 ± 0.2 nmol/L; < 0.001). Neither of the two ELISAs detected any significant difference in plasma phylloquinone concentrations between depletion and supplementation (ELISA A, = 0.76; ELISA B, = 0.29). These findings reinforce the need to validate plasma phylloquinone assays as they become available. 2023;x:xx.
用于测量循环叶绿醌的酶联免疫吸附测定（ELISA）已实现商业化，但它们的有效性尚不确定。本研究的目的是比较在一项消耗（约10微克叶绿醌/天）-补充（约500微克叶绿醌/天）研究中，使用两种商用ELISA测量的108份样本的血浆叶绿醌浓度与使用经过验证的高效液相色谱（HPLC）测定法测量的浓度。用ELISA A测量的血浆叶绿醌几何平均值为0.70纳摩尔/升，比用HPLC测量的值低37%。ELISA B测量的平均值为12.4纳摩尔/升，比HPLC测量值高700%以上。在叶绿醌消耗期间，使用HPLC测量的血浆叶绿醌显著低于补充期间（0.4±0.1与1.2±0.2纳摩尔/升相比；P<0.001）。两种ELISA均未检测到消耗和补充之间血浆叶绿醌浓度的任何显著差异（ELISA A，P = 0.76；ELISA B，P = 0.29）。这些发现强化了在血浆叶绿醌测定法可用时对其进行验证的必要性。2023；x:xx。
