Huertas Raul, Torres-Jerez Ivone, Curtin Shaun J, Scheible Wolf, Udvardi Michael
Noble Research Institute LLC, Ardmore, OK, United States.
United States Department of Agriculture, Plant Science Research Unit, St. Paul, MN, United States.
Front Plant Sci. 2023 Jun 13;14:1211107. doi: 10.3389/fpls.2023.1211107. eCollection 2023.
Three -like genes encoding putative ubiquitin-conjugating E2 enzymes of were characterized for potential roles in phosphorous (P) homeostasis and symbiotic nitrogen fixation (SNF). All three genes, , contain miR399-binding sites characteristic of genes in other plant species. Distinct spatiotemporal expression patterns and responsiveness of gene expression to P- and N-deprivation in roots and shoots indicated potential roles, especially for , in P and N homeostasis. Phenotypic analysis of mutants revealed that MtPHO2B is integral to Pi homeostasis, affecting Pi allocation during plant growth under nutrient-replete conditions, while MtPHO2C had a limited role in controlling Pi homeostasis. Genetic analysis also revealed a connection between Pi allocation, plant growth and SNF performance. Under N-limited, SNF conditions, Pi allocation to different organs was dependent on MtPHO2B and, to a lesser extent, MtPHO2C and MtPHO2A. MtPHO2A also affected Pi homeostasis associated with nodule formation. Thus, genes play roles in systemic and localized, i.e., nodule, P homeostasis affecting SNF.
对编码假定泛素结合E2酶的三个类似基因进行了表征，以研究它们在磷（P）稳态和共生固氮（SNF）中的潜在作用。所有这三个基因都含有其他植物物种中类似基因特有的miR399结合位点。根和地上部中基因表达的不同时空表达模式以及对P和N缺乏的反应表明了它们的潜在作用，特别是对于在P和N稳态中的作用。对突变体的表型分析表明，MtPHO2B对于磷稳态不可或缺，在营养充足条件下植物生长期间影响磷的分配，而MtPHO2C在控制磷稳态中的作用有限。遗传分析还揭示了磷分配、植物生长和SNF性能之间的联系。在N限制的SNF条件下，磷向不同器官的分配取决于MtPHO2B，在较小程度上取决于MtPHO2C和MtPHO2A。MtPHO2A也影响与根瘤形成相关的磷稳态。因此，这些基因在系统和局部（即根瘤）磷稳态中发挥作用，影响SNF。
